The interplay between bone and ex vivo-expanded gamma delta T cells.
| Project/Area Number |
26861583
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Osaka Dental University |
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Principal Investigator |
DOMAE Eisuke 大阪歯科大学, 歯学部, 講師 (50454559)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | γδ T cells / IL-12 / IL-18 / STAT4 / NF-κB / IκBζ / γδT細胞 / I kappa B zeta / NF-kappa B / BTLA / Vγ9Vδ2T細胞 / 骨芽細胞 |
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| Outline of Final Research Achievements |
We examined the effects of inflammatory environment on ex-vivo expanded Vγ9Vδ2 T cells. Vγ9Vδ2 T cells treated with IL-12 and IL-18 enhanced effector functions, including the expression of IFN-γ and granzyme B, and cytotoxicity. These enhanced effector responses following IL-12 and IL-18 treatment were associated with homotypic aggregation, enhanced expression of ICAM-1 and decreased expression of the B- and T-lymphocyte attenuator (BTLA), a co- inhibitory receptor. IL-12 and IL-18 also induced the antigen-independent proliferation of Vγ9Vδ2 T cells. Increased expression of IκBζ, IL-12Rβ2 and IL-18Rα following IL-12 and IL-18 stimulation resulted in sustained activation of STAT4 and NF-κB. Thus, we showed that IL-12 and IL-18 synergize to activate human ex vivo-expanded Vγ9Vδ2 T cells.
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Report
(4 results)
Research Products
(2 results)
