Establishment of the isolation method of odontoblasts and clarification of the mechanism of the dentinal tuble formation.

Research Project

Project/Area Number	26861600
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Conservative dentistry
Research Institution	Nagasaki University
Principal Investigator	NAKAJIMA Kazunori 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (40707246)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000) Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	象牙芽細胞 / Tie2
Outline of Final Research Achievements	Dentin is located inside of enamal and added by foreign stimulation throughout our life by odonoblastst. Because odontoblasts have not been isolated so far, their biological features were not known well. The aim of this study was to isolate odontoblasts from pulp tissues by targeting the specific molecule expressed by odontoblasts. As a results, odontoblasts were isolated , however, it was suggested that their long-time culture required additional certain factors to maintain their biological features. This findings may contribute ti the development of the researchs of odontoblasts cell biology.