• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of new treatments for radicular cyst by inducing apoptosis in cultured porcine epithelial cell rests of Malassez

Research Project

Project/Area Number 26861606
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Conservative dentistry
Research InstitutionTokyo Dental College

Principal Investigator

AIDA NATSUKO  東京歯科大学, 歯学部, 講師 (90615379)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Keywords歯内療法学 / マラッセ上皮遺残 / アポトーシス / jasplakinolide / Jasplakinolide
Outline of Final Research Achievements

Cell activity, apoptosis and change in cell shape applying Jasplakinolide on porcine epithelial cell rests of Malassez, apoptosis were researched. ERM derived from porcine were spread in a 96-well dish using Dulbecco’s modified Eagle’s medium. The actin-specific stabilization reagent, jasplakinolide, was incorporated into the culture medium and incubated for 24 h. To evaluate cell viability, the WST-1 assay was carried out and absorption (450 nm) was measured. To detect apoptotic cells, monoclonal antibody to single-strand DNA was used and absorption (405 nm) was measured.
Cell viability decreased in a concentration dependent manner, and cell viability in the jasplakinolide-treated ERM was lower than that in nontreated ERM. Apoptotic cells in the jasplakinolide-treated ERM were more frequently detected compared to that in nontreated ERM. Actin stabilization by jasplakinolide inhibited cell viability and induced apoptosis in epithelial cell rests of Malassez.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] Actin Stabilization Induces Apoptosis in Cultured Porcine Epithelial Cell Rests of Malassez.2016

    • Author(s)
      Aida N, Ushikubo T, Kobayashi F, Sako R, Suehara M, Furusawa M, Muramatsu T.
    • Journal Title

      International Endodontics Journal

      Volume: 49(7) Issue: 7 Pages: 663-669

    • DOI

      10.1111/iej.12494

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] Jasplakinolide Induces Apoptosis in Epithelial Cell Rests of Malasse2015

    • Author(s)
      Takashi Muramatsu
    • Organizer
      93th general session IADR
    • Place of Presentation
      Boston
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Research-status Report
  • [Presentation] JasplakinolideがMalassez上皮遺残細胞に及ぼす影響2014

    • Author(s)
      小林史枝
    • Organizer
      東京歯科大学学会 第298回
    • Place of Presentation
      東京都千代田区
    • Year and Date
      2014-10-18 – 2014-10-19
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi