| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
|
|---|
| Outline of Final Research Achievements |
Cell activity, apoptosis and change in cell shape applying Jasplakinolide on porcine epithelial cell rests of Malassez, apoptosis were researched. ERM derived from porcine were spread in a 96-well dish using Dulbecco’s modified Eagle’s medium. The actin-specific stabilization reagent, jasplakinolide, was incorporated into the culture medium and incubated for 24 h. To evaluate cell viability, the WST-1 assay was carried out and absorption (450 nm) was measured. To detect apoptotic cells, monoclonal antibody to single-strand DNA was used and absorption (405 nm) was measured.
Cell viability decreased in a concentration dependent manner, and cell viability in the jasplakinolide-treated ERM was lower than that in nontreated ERM. Apoptotic cells in the jasplakinolide-treated ERM were more frequently detected compared to that in nontreated ERM. Actin stabilization by jasplakinolide inhibited cell viability and induced apoptosis in epithelial cell rests of Malassez.
|
