Protease activity-independent role of BMP-1 in reparative dentin formation
| Project/Area Number |
26861609
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservative dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 修復象牙質 / BMP-1 / CCN2/CTGF / 象牙質・歯髄複合体 |
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| Outline of Final Research Achievements |
In this study, we demonstrated that the expression of bone morphogenetic protein-1 (BMP-1), CCN family member 2/connective tissue growth factor (CCN2/CTGF), and dentin sialophosphoprotein (DSPP) was accelerated in the layer of odontoblast-like cells and reparative dentin subjacent dental caries in human teeth. BMP-1 induced the expression of CCN2/CTGF independently of protease activity in the human dental pulp cells but not that of DSPP or dentin matrix protein-1 (DMP-1). Furthermore, cell membrane glycosylation was altered by BMP-1 stimulation as analyzed by lectin microarray.
These findings indicate that a novel property of BMP-1 which potentially enhances bone-like reparative dentin formation through CCN2 expression and membrane glycosylation in dentin-pulp complex.
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Report
(3 results)
Research Products
(9 results)
