| Project/Area Number |
26861671
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
OHYAMA Yoshio 東京医科歯科大学, 大学院医歯学総合研究科, 非常勤講師 (30451975)
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| Research Collaborator |
KANEMARU Tomoki
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 骨造成 / 細胞外タンパク質 / システインノットプロテイン / システインリッチドメイン / BMP-2 / Vwc2 / Vwc2-like / 国際情報交換 |
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| Outline of Final Research Achievements |
The ALP activity of mouse primary osteoblasts and MC3T3-E1 cells cultured for 72 hours in the presence of Vwc2 protein was significantly increased compared with that in the absence. In addition, when MC3T3-E1 cells were cultured in the presence of Vwc2 protein for 3 weeks, there was a significant increase in mineralization compared with those cultured in the absence. Furthermore, expression of osteoblast differentiation marker was significantly increased in mRNA collected from primary mouse cells cultured in the presence of Vwc2 protein. Even in the in vivo experiments, a significant increase in osteogenesis was observed in the Vwc 2 group as compared with the negative control group, and a significant increase in calcification rate was observed.
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