Study on elucidation of calcification promoting mechanism by Vwc2-like and clinical application of Vwc2-like
Project/Area Number |
26861671
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Dental engineering/Regenerative dentistry
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
OHYAMA Yoshio 東京医科歯科大学, 大学院医歯学総合研究科, 非常勤講師 (30451975)
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Research Collaborator |
KANEMARU Tomoki
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 骨造成 / 細胞外タンパク質 / システインノットプロテイン / システインリッチドメイン / BMP-2 / Vwc2 / Vwc2-like / 国際情報交換 |
Outline of Final Research Achievements |
The ALP activity of mouse primary osteoblasts and MC3T3-E1 cells cultured for 72 hours in the presence of Vwc2 protein was significantly increased compared with that in the absence. In addition, when MC3T3-E1 cells were cultured in the presence of Vwc2 protein for 3 weeks, there was a significant increase in mineralization compared with those cultured in the absence. Furthermore, expression of osteoblast differentiation marker was significantly increased in mRNA collected from primary mouse cells cultured in the presence of Vwc2 protein. Even in the in vivo experiments, a significant increase in osteogenesis was observed in the Vwc 2 group as compared with the negative control group, and a significant increase in calcification rate was observed.
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Report
(5 results)
Research Products
(25 results)
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[Journal Article] Ellis van Creveld2 is required for postnatal craniofacial bone development.2016
Author(s)
Badri MK, Zhang H, Ohyama Y, Venkitapathi S, Kamiya N, Takeda H, Ray M, Scott G, Tsuji T, Kunieda T, Mishina Y, Mochida Y.
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Journal Title
Anatomical record (Hoboken, N.J. : 2007)
Volume: 2016 Aug;299(8)
Issue: 8
Pages: 1110-20
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] NOTCH3 Is Induced in Cancer-Associated Fibroblasts and Promotes Angiogenesis in Oral Squamous Cell Carcinoma.2016
Author(s)
Kayamori K, Katsube K, Sakamoto K, Ohyama Y, Hirai H, Yukimori A, Ohata Y, Akashi T, Saitoh M, Harada K, Harada H, Yamaguchi A.
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Journal Title
PLoS One.
Volume: Apr 28;11(4
Related Report
Peer Reviewed / Open Access
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[Journal Article] Expression of Evc2 in craniofacial tissues and craniofacial bone defects in Evc2 knockout mouse.2016
Author(s)
Badri MK, Zhang H, Ohyama Y, Venkitapathi S, Alamoudi A, Kamiya N, Takeda H, Ray M, Scott G, Tsuji T, Kunieda T, Mishina Y, Mochida Y.
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Journal Title
Archives of Oral Biology
Volume: 2016 Aug
Pages: 142-52
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] FAM20A binds to and regulates FAM20C localization.2016
Author(s)
Ohyama Y, Lin JH, Govitvattana N, Lin IP, Venkitapathi S, Alamoudi A, Husein D, An C, Hotta H, Kaku M, Mochida Y.
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Journal Title
Scientific Reports.
Volume: 13;6:27784.
Issue: 1
Pages: 27784-27784
DOI
Related Report
Peer Reviewed / Open Access
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