Application of direct reprogramming technology to periodontal tissue Regenerative therapy
| Project/Area Number |
26861679
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Yamamoto Kenta 京都府立医科大学, 医学(系)研究科(研究院), 助教 (00636160)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 再生医療 / 骨芽細胞 / ダイレクトリプログラミング |
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| Outline of Final Research Achievements |
Alveolar bone resorption associated with advanced periodontitis causes tooth loss to remarkably reduce patients’ QOL. Transplantation of autologous osteoblasts into the alveolar bone legion may facilitate alveolar bone regeneration to prevent tooth loss. Recently we reported that human fibroblasts were directly reprogrammed into osteoblasts by transducing four transcription factor genes by means of retrovirus vectors. In this study, we improved this technology to obtain reprogrammed osteoblasts in which any transgene is not integrated into their chromosomes.
As results, human fibroblasts were successfully converted into osteoblasts by transfecting genes by a plasmid vector. Transgenes were not integrated into the chromosome of the resultant cells. The present technology may lead to novel regenerative medicine for alveolar bone resorption.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Transduction of Oct6 or Oct9 gene concomitant with Myc family gene induced osteoblast-like phenotypic conversion in normal human fibroblast.2015
Author(s)
Mizoshiri N, Kishida T, Yamamoto K, Shirai T, Terauchi R, Tsuchida S, Mori Y, Ejima A, Sato Y, Arai Y, Fujiwara H, Yamamoto T, Kanamura N, Mazda O, Kubo T.
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Journal Title
Biochem Biophys Res Commun
Volume: 467
Issue: 4
Pages: 1110-1116
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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