| Budget Amount *help |
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
We aimed to develop a novel bone grafting material. We isolated peripheral blood mononuclear cells (PBMCs) from venous blood taken from the front legs of male mongrel adult dogs by means of gradient centrifugation (Ficoll group) or nonwoven filtering (Filter group), and mixed and incubated these cells with β-TCP microgranules to adhere to the surface of β-TCP granules. We then grafted the PBMCs/β-TCP granules into experimentally prepared bone defects in dog mandibles. A tendency toward greater osteogenesis was recognized in the Ficoll and Filter groups compared with the control group. Furthermore, the procedure for the filtering method is simple and may be applicable for dental graft treatment in general dental clinics. Taken together, PBMCs harvested using a nonwoven filter and incubated with β-TCP granules may provide a useful material for regenerating periodontal tissue and bone.
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