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Human dental pulp cell-derived spheroids and traditional Chinese medicine for central nerve system regeneration

Research Project

Project/Area Number 26861689
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Dental engineering/Regenerative dentistry
Research InstitutionThe Nippon Dental University

Principal Investigator

Xiao Li  日本歯科大学, 生命歯学部, 講師 (80548256)

Research Collaborator Miwa Nobuhiko  県立広島大学, 生命環境学部, 名誉教授 (00142141)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords歯髄細胞 / 中枢神経再生 / スフェロイド / 海馬組織 / 神経新生 / 歯髄由来細胞 / 神経分化能 / 共培養 / HaCaT細胞 / 歯髄幹細胞 / 神経再生 / 器官発生
Outline of Final Research Achievements

Mammalian adult central nerve system (CNS) injuries are devastating because of the intrinsic difficulties for effective neuronal regeneration. The greatest problem to be overcome for CNS recovery is the poor regeneration of neuronal cells. Because of the harsh micro-environment, it is very difficult to regenerate the damaged neuronal cells, especially neurons. In this study, we made spheroids from human dental pulp-derived cells. The spheroids were able to survive under very harsh environment, such as shortage of oxygen and nutrition. When they were cultivated in neuronal inductive medium, they could be differentiated into neuronal cells. Patch clamp technique indicated that cells derived from the spheroids exhibited the capacity to produce a tetrodotoxin-sensitive (membrane) depolarization suggesting they have potential to be differentiated into functional neurons. The spheroids also improved neuronal cells regeneration in co-cultivated mouse hippocampus slices in vitro.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2017 2016 2015 2014

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (3 results)

  • [Journal Article] The Lipophilic Vitamin C Derivative, 6-O-Palmitoylascorbate Protects Human Keratinocytes and 3D-Human Skin Equivalents Against X-Ray-Induced Oxidative Stress and Apoptosis More Markedly Than L-Ascorbic Acid.2017

    • Author(s)
      Xiao L, Miwa N.
    • Journal Title

      J Cell Biochem.

      Volume: 118 Issue: 2 Pages: 318-329

    • DOI

      10.1002/jcb.25639

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cover Image2017

    • Author(s)
      Xiao L, Miwa N.
    • Journal Title

      J Cell Biochem

      Volume: 118 Issue: 2 Pages: 1-1

    • DOI

      10.1002/jcb.25848

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hydrogen-rich water achieves cytoprotection from oxidative stress injury in human gingival fibroblasts in culture or 3D-tissue equivalents, and wound-healing promotion, together with ROS-scavenging and relief from glutathione diminishment.2016

    • Author(s)
      Xiao L, Miwa N.
    • Journal Title

      Hum Cell

      Volume: 30 Issue: 2 Pages: 72-87

    • DOI

      10.1007/s13577-016-0150-x

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] From regenerative dentistry to regenerative medicine: progress, challenges, and potential applications of oral stem cells.2014

    • Author(s)
      Xiao L, Nasu M.
    • Journal Title

      Stem Cells Cloning.

      Volume: 7 Pages: 89-99

    • DOI

      10.2147/sccaa.s51009

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Stem cell therapy for central nerve system injuries: glial cells hold the key.2014

    • Author(s)
      Xiao L, Saiki C, Ide R.
    • Journal Title

      Neural Regen Res.

      Volume: 9 Issue: 13 Pages: 1253-1260

    • DOI

      10.4103/1673-5374.137570

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cell death, cavitation and spontaneous multi-differentiation of dental pulp stem cells-derived spheroids in vitro: a journey to survival and organogenesis.2014

    • Author(s)
      Xiao L, Kumazawa Y, Okamura H.
    • Journal Title

      Biol Cell.

      Volume: 106 Issue: 12 Pages: 405-419

    • DOI

      10.1111/boc.201400024

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Protective effects of hydrogen-rich water on free radical initiators-induced oxidative stress injuries in human gingival fibroblasts and three-dimensional gingival tissue equivalents2016

    • Author(s)
      肖 黎、三羽 信比古
    • Organizer
      第58回歯科基礎医学会学術大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Dental pulp cells-derived spheroids differentiated into functional neuronal cells under three-dimensional culture conditions2015

    • Author(s)
      Li Xiao*, Chikako Saiki, Ryoji Ide
    • Organizer
      第57回歯科基礎基礎医学会学術大会
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Research-status Report
  • [Presentation] Spontaneous multi-differentiation of dental pulp stem cells-derived spheroids on matrigel: A journey to survival and organogenesis2014

    • Author(s)
      Xiao L, Kumazawa Y, Okamura H.
    • Organizer
      第56回歯科基礎医学会学術大会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2014-09-26
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-07  

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