Regeneration of the tooth and periodontal tissue unit derived from adult tooth cells, in vitro.

• Project/Area Number: 26861690
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: toyomura junko 日本歯科大学, 生命歯学部, 助教 (80645630)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 歯の再生

Outline of Final Research Achievements

We aimed to generate correct tooth structure by adult tooth cells derived from deciduous tooth for clinical application.
We isolated pig epithelial cell rests of Malassez(pERM),human dental pulp cells(hDPcs) and human periodontal ligament cells(hPDLcs).We made atelocollagen beads included hDPcs and attached with pERM(we call cell-bead). Cell-bead was wrapped in hPDLcs-sheet and was transplanted in abdominal cavity of scid mouse.We performed an operation one month later and found the tooth and periodontal tissue unit-like structure under a stereoscopic microscope.

Research Products

• [Journal Article] Establishment and characterization of a clear cell carcinoma cell line, designated NOCC, derived from human ovary (2016)
  • Author(s): Ohyama A, Toyomura J, Tachibana T, Isonishi S, Takahashi H, Ishikawa H
  • Journal Title: Human Cell Volume: 29 Issue: 4 Pages: 188-196
  • DOI: 10.1007/s13577-016-0142-x
  • Peer Reviewed
• [Journal Article] Isolation and characterization of embryonic ameloblast lineage cells derived from tooth buds of fetal miniature swine (2016)
  • Author(s): ２.Nakahara T, Tominaga N, Toyomura J, Tachibana T, Ide Y, Ishikawa H.
  • Journal Title: In Vitro Cell Dev Biol Anim Volume: 52 Issue: 4 Pages: 445-453
  • DOI: 10.1007/s11626-015-9987-7
  • Peer Reviewed
• [Presentation] Tooth-derived Hepatocytes Transplantation to Swine Liver Failure (2017)
  • Author(s): Yagi H, Yokoyama T, Tanaka T, Toyomura J, Yaegaki K, Ishikawa H
  • Organizer: IADR 95nd
  • Place of Presentation: San Francisco(America)
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] 外胚葉性間葉であるヒト頬脂肪体幹細胞から誘導分化させた神経系細胞によるパーキンソン病治療 (2017)
  • Author(s): 高橋悠, 大山晃弘, 畑-川上未有希, 豊村順子, 石川博, 田中彰
  • Organizer: 第16回日本再生医療学会総会
  • Place of Presentation: 仙台国際センター(宮城県)
  • Year and Date: 2017-03-07
• [Presentation] ヒト歯髄細胞を用いた神経および微細血管網を含む３次元骨組織形成 (2017)
  • Author(s): 渡邊美穂, 大山晃弘, 豊村順子, 石川博, 田中彰
  • Organizer: 第16回日本再生医療学会総会
  • Place of Presentation: 仙台国際センター(宮城県)
  • Year and Date: 2017-03-07
• [Presentation] ミニブタ胎仔の乳臼歯歯冠由来エナメル芽細胞株におけるグリコーゲンの解析 (2016)
  • Author(s): 富永徳子, 豊村順子, 石川博, 中原貴
  • Organizer: 第61回日本口腔外科学会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2016-11-25
• [Presentation] 歯髄幹細胞から培養下に作る再生骨の移植時期，特に VEGF と HIF-1 αとの関連 (2016)
  • Author(s): 大山晃弘, 豊村順子, 渡邊美穂, 田中彰, 石川博
  • Organizer: 第61回日本口腔外科学会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2016-11-25
• [Presentation] ヒト頬脂肪体由来幹細胞より分化誘導した神経系細胞移植によるパーキンソン病治療 (2016)
  • Author(s): 高橋 悠, 大山晃弘, 豊村順子, 畑-川上未有希, 石川博, 田中彰
  • Organizer: 第34回日本ヒト細胞学会学術集会
  • Place of Presentation: 東大寺総合文化センター(奈良県)
  • Year and Date: 2016-07-16
• [Presentation] 低酸素下と通常酸素下でヒト歯髄幹細胞を神経、骨、軟骨に分化誘導する際のHIF-1αとVEGFの産生パターンの比較 (2016)
  • Author(s): 大山晃弘, 豊村順子, 渡邊美穂, 石川博
  • Organizer: 第34回日本ヒト細胞学会学術集会
  • Place of Presentation: 東大寺総合文化センター(奈良県)
  • Year and Date: 2016-07-16