| Project/Area Number |
26861701
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
|
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| Research Institution | Chiba University (2015)
University of Tsukuba (2014) |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | exosome / microRNA / 口腔がん / 臨床腫瘍学 / エキソソーム |
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| Outline of Final Research Achievements |
In the lipid bilayer of exosomes, proteins or nucleic acids such as microRNA are included and take intercellular signaling. We extracted microRNAs derived from exosomes in fluid and analyzed the expression of miR-205-5p and miR-200c-3p. In comparison with the cell line derived from metastatic oral cancer, miR-205-5p and miR-200c-3p showed a decreased expression in the cells, but showed an increased expression in the exosomes. In comparison with the healthy controls, the expression of miR-205-5p was significantly down-regulated in the saliva of oral cancer. In summary, exosomes derived from these cancer cells affected the neighboring cancer cells, and likelihood to make the environment that was easy to metastasize are suggested.
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