Oral cancer intraoperative detection by fluorescent probe.

• Project/Area Number: 26861710
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Shinshu University
• Principal Investigator: SHIMANE Tetsu 信州大学, 医学部附属病院, 特任研究員 (80714469)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: GGT / 口腔癌 / 扁平上皮癌 / アミノ酸代謝 / 術中診断 / γ-Glu / グルタミル基 / 蛍光 / 迅速診断 / in vivo / 口腔がん / SCC / ｇGlu-HMRG / 口腔扁平上皮癌

Outline of Final Research Achievements

The ability of the unaided human eye to detect accurate borders between cancer and normal tissue during surgery is limited. The aim of this study was to evaluate the performance of g-Glu-HMRG in distinguishing oral squamous cell carcinoma (OSCC) from normal tissue. After removing OSCC, g-Glu-HMRG was sprayed on the surface of fresh sliced section of OSCCs. Fluorescent images were obtained by in vivo imager. Fluorescence was firstly confirmed at invasive fronts of OSCC 1 to 3 minutes after the spraying. 25 minutes after the spraying, we could distinguish whole OSCC invasive fronts from normal tissue. In conclusion, the results of this study demonstrated that g-Glu-HMRG could rapidly detect OSCC invasive front. Instant activation of the probe makes it feasible for incorporation into surgical intraoperative resection procedures.

Research Products

• [Journal Article] Oral cancer intraoperative detection by topically spraying a γ-glutamyl transpeptidase-activated fluorescent probe. (2016)
  • Author(s): Shimane T, Aizawa H, Koike T, Kamiya M, Urano Y, Kurita H.
  • Journal Title: Oral oncology Volume: 54 Pages: e16-e18
  • DOI: 10.1016/j.oraloncology.2015.12.003
  • Peer Reviewed
• [Journal Article] Grainyhead-like 2 regulates epithelial plasticity and stemness in oral cancer cells. (2016)
  • Author(s): Chen W, Yi JK, Shimane T, Mehrazarin S, Lin YL, Shin KH, Kim RH, Park NH, Kang MK.
  • Journal Title: Carcinogenesis Volume: 1
  • Peer Reviewed
• [Journal Article] Construction and characterization of human oral mucosa equivalent using hyper-dry amniotic membrane as a matrix. (2016)
  • Author(s): Qi Fangfang, Toshiko Yoshida, Takeshi Koike, Hitoshi Aizawa, Tetsu Shimane, Yinghui Li, Shinichi Yamada, Motonori Okabe, Toshiko Nikaido, Hiroshi Kurita.
  • Journal Title: Archives of Oral Biology Volume: 65 Pages: 26-34
  • DOI: 10.1016/j.archoralbio.2016.01.011
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Predictability of staged localized alveolar ridge augmentation using a micro titanium mesh. (2015)
  • Author(s): Uehara S, Kurita H, Shimane T, Sakai H, Kamata T, Teramoto Y, Yamada S.
  • Journal Title: Oral and maxillofacial surgery Volume: 19 Issue: 4 Pages: 411-6
  • DOI: 10.1007/s10006-015-0513-6
  • Peer Reviewed
• [Presentation] 蛍光プローブによる口腔扁平上皮癌深部断端の検出における有用性の検討 (2014)
  • Author(s): 嶋根哲
  • Organizer: 第59回日本口腔外科学会総会・学術大会
  • Place of Presentation: 千葉市(幕張メッセ)
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] Oral Cancer Detection by New Concept Probe (2014)
  • Author(s): 嶋根哲
  • Organizer: 96th AAOMS Annual Meeting ; American Association of Oral and Maxillofacial Surgeons
  • Place of Presentation: Honolulu（USA）
  • Year and Date: 2014-09-08 – 2014-09-13
• [Presentation] 酵素活性検出蛍光プローブによる口腔癌の検出 (2014)
  • Author(s): 嶋根哲
  • Organizer: 第52回日本癌治療学会学術集会
  • Place of Presentation: 横浜市(パシフィコ横浜）
  • Year and Date: 2014-08-28 – 2014-08-30