| Project/Area Number |
26861723
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 歯学 / 再生医学 / 幹細胞 / 遺伝子疾患 / 細胞培養 / 歯髄細胞 / 分化誘導 / 遺伝性疾患 / 人工多能性幹細胞 |
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| Outline of Final Research Achievements |
Human pluripotent stem cells hold great promise for their practical and scientific potentials. To improve understanding of self-renewal and differentiation, we could generate and maintain human iPSCs in serum- and feeder-free culture conditions using retroviral vectors. To avoid the unpredictable side effects associated with retrovirus integration, we generated hiPSCs from dental pulp cells and PBMCs with a non-integrating replication-defective and persistent Sendai virus (SeVdp) vector. Using this system, pluripotent and self-renewing hiPSCs could be easily and stably generated and propagated. With this system we established hiPSCs from cleidocranial dysplasia (CCD) caused by a mutation of RUNX2, which has an important role in the differentiation of osteoblasts and maturation of chondrocytes. The cartilage in the teratomas of CCD-iPSCs showed abnormalities. These CCD-iPSCs would be beneficial to clarify the molecular mechanism and for development of medical applications.
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