Generation and analysis of oral and maxillofacial disease-specific human iPSCs in completely serum-, feeder-, and integration-free culture
| Project/Area Number |
26861723
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 歯学 / 再生医学 / 幹細胞 / 遺伝子疾患 / 細胞培養 / 歯髄細胞 / 分化誘導 / 遺伝性疾患 / 人工多能性幹細胞 |
|---|
| Outline of Final Research Achievements |
Human pluripotent stem cells hold great promise for their practical and scientific potentials. To improve understanding of self-renewal and differentiation, we could generate and maintain human iPSCs in serum- and feeder-free culture conditions using retroviral vectors. To avoid the unpredictable side effects associated with retrovirus integration, we generated hiPSCs from dental pulp cells and PBMCs with a non-integrating replication-defective and persistent Sendai virus (SeVdp) vector. Using this system, pluripotent and self-renewing hiPSCs could be easily and stably generated and propagated. With this system we established hiPSCs from cleidocranial dysplasia (CCD) caused by a mutation of RUNX2, which has an important role in the differentiation of osteoblasts and maturation of chondrocytes. The cartilage in the teratomas of CCD-iPSCs showed abnormalities. These CCD-iPSCs would be beneficial to clarify the molecular mechanism and for development of medical applications.
|
Report
(3 results)
Research Products
(16 results)
-
[Journal Article] Generation of cleidocranial dysplasia-specific human induced pluripotent stem cells in completely serum-, feeder-, and integration-free culture.2015
Author(s)
Yamasaki S, Hamada A, Akagi E, Nakatao H, Ohtaka M, Nishimura K, Nakanishi M, Toratani S, Okamoto T.
-
Journal Title
In Vitro Cell Dev Biol Anim.
Volume: 52
Issue: 2
Pages: 252-264
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
[Presentation] Generation and maintenance of integration-free human induced pluripotent stem (hiPS) cells from peripheral blood mononuclear cells in serum- and feeder-free growth factor defined medium2015
Author(s)
Hirotaka NAKATAO, Sachiko YAMASAKI, Eri AKAGI, Atsuko HAMADA, Manami OHTAKA, Ken NISHIMURA, Mahito NAKANISHI, Shigeaki TORATANI, Tetsuji OKAMOTO
Organizer
2015 in vitro biology biology meeting
Place of Presentation
Tucson, Arizona,USA
Year and Date
2015-06-03
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
