Elucidation of the morphological change and resorption of bone mechanism in the ameloblastoma
| Project/Area Number |
26861732
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
imai yuko 九州大学, 大学病院, 助教 (30592688)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | ameloblastoma / RANKL / TRP channel / エナメル上皮腫 / V-ATPase / CLC-7 / 骨溶解 |
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| Outline of Final Research Achievements |
Mechanisms of proliferation and differentiation in ameloblastoma, an odontogenic tumor, remain unclear. We previously reported that AM-1, a cell line of plexiform ameloblastoma, resorbed peritumoral bone by release of H+ from V-ATPase and Cl- from ClC-7 on its plasma membrane. Moreover, we found that increasing extracellular Ca2+ concentration evoked morphological change and that administration of RANKL facilitated proliferation. Therefore, we aimed to clarify these mechanisms. We performed RT-PCR, Western blot, immunohistochemistry and cell counting using several modulators of RANK receptor signaling cascade and Ca2+ permeable channels. Our results show that TRPV2, V3, V4 and TRPM7 are candidates for Ca2+ influx channels, and that RANKL-induced facilitation of proliferation was caused by upregulation and dephosphorylation of NFATc1 and c2.
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Report
(5 results)
Research Products
(17 results)
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[Journal Article] Surface vacuolar ATPase in ameloblastoma contributes to tumor invasion of the jaw bone.2016
Author(s)
Yoshimoto S, Morita H, Matsubara R, Mitsuyasu T, Imai Y, Kajioka S, Yoneda M, Ito Y, Hirofuji T, Nakamura S, Hirata M.
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Journal Title
International Journal of Oncology
Volume: 48 (3)
Issue: 3
Pages: 1258-1270
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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