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Quiescent tumor cell models for regulating tumor dormancy

Research Project

Project/Area Number 26861735
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Surgical dentistry
Research InstitutionKumamoto University (2015)
Hamamatsu University School of Medicine (2014)

Principal Investigator

Shinriki Satoru  熊本大学, 大学院生命科学研究部, 講師 (00583048)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords静止期癌細胞 / 骨髄播種癌細胞 / CYLD / 抗癌剤耐性
Outline of Final Research Achievements

Suppression of CYLD expression by siRNA in head and neck squamous cell carcinoma (HNSCC) cell lines led to an increase in G0 phase. Although, to establish a quiescent tumor cell model, we utilized Tet-on shRNA to regulate CYLD expression, the knockdown efficacy has been insufficient. Therefore, further investigation is needed.
Besides, we established a dormant bone marrow-disseminated tumor cell (BM-DTC)-derived subclone using OSCC cell line, HEp3. Using this subclone, we reported that intrinsic TGF-β2-SDF-1-CXCR4 signaling was important for slow-cycling state and drug resistance in dormant BM-DTC.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (8 results)

All 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (5 results)

  • [Journal Article] Intrinsic TGF-β2-triggered SDF-1-CXCR4 signaling axis is crucial for drug resistance and a slow-cycling state in bone marrow-disseminated tumor cells.2015

    • Author(s)
      Nakamura T, Shinriki S, Jono H, Guo J, Ueda M, Hayashi M, Yamashita S, Zijlstra A, Nakayama H, Hiraki A, Shinohara M, Ando Y.
    • Journal Title

      Oncotarget

      Volume: 6 Pages: 1008-1019

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Osteopontin-integrin α(v)β(3) axis is crucial for 5-fluorouracil resistance in oral squamous cell carcinoma.2015

    • Author(s)
      Nakamura T, Shinriki S, Jono H, Ueda M, Nagata M, Guo J, Hayashi M, Yoshida R, Ota T, Ota K, Kawahara K, Nakagawa Y, Yamashita S, Nakayama H, Hiraki A, Shinohara M, Ando Y.
    • Journal Title

      FEBS Lett

      Volume: 589 Issue: 2 Pages: 231-239

    • DOI

      10.1016/j.febslet.2014.12.004

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hypoxia suppresses cylindromatosis (CYLD) expression to promote inflammation in glioblastoma: possible link to acquired resistance to anti-VEGF therapy.2014

    • Author(s)
      Guo J, Shinriki S, Su Y, Nakamura T, Hayashi M, Tsuda Y, Murakami Y, Tasaki M, Hide T, Takezaki T, Kuratsu J, Yamashita S, Ueda M, Li JD, Ando Y, Jono H.
    • Journal Title

      Oncotarget

      Volume: 5 Pages: 6353-6364

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 骨髄播種癌細胞の増殖抑制状態と抗癌剤耐性への細胞自律的制御機構の関与2015

    • Author(s)
      神力 悟
    • Organizer
      第62回日本臨床検査医学会学術集会
    • Place of Presentation
      長良川国際会議場、岐阜
    • Year and Date
      2015-11-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] 骨髄播種癌細胞が自律的に誘導する増殖抑制状態と抗癌剤耐性2015

    • Author(s)
      神力 悟
    • Organizer
      第55回日本臨床化学会年次学術集会
    • Place of Presentation
      大阪大学、大阪
    • Year and Date
      2015-10-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] Intrinsic molecular characteristics of bone marrow-disseminated tumor cells2015

    • Author(s)
      神力 悟
    • Organizer
      第74回日本癌学会学術集会
    • Place of Presentation
      名古屋国際会議場、名古屋
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Loss of CYLD promotes cell migration by inhibiting Smurf2-dependent ALK5 degradation in oral squamous cell carcinoma.2014

    • Author(s)
      神力悟
    • Organizer
      第19回静岡健康・長寿学術フォーラム
    • Place of Presentation
      プラサ ヴェルテ(ふじのくに千本松フォーラム), 沼津
    • Year and Date
      2014-11-07 – 2014-11-08
    • Related Report
      2014 Research-status Report
  • [Presentation] CYLDの発現低下はSmurf2依存的なALK5の分解を阻害することで口腔扁平上皮癌の遊走を促進させる2014

    • Author(s)
      神力悟
    • Organizer
      第73回日本癌学会学術集会
    • Place of Presentation
      パシフィコ横浜, 横浜
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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