| Project/Area Number |
26861735
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Kumamoto University (2015)
Hamamatsu University School of Medicine (2014) |
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Principal Investigator |
Shinriki Satoru 熊本大学, 大学院生命科学研究部, 講師 (00583048)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 静止期癌細胞 / 骨髄播種癌細胞 / CYLD / 抗癌剤耐性 |
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| Outline of Final Research Achievements |
Suppression of CYLD expression by siRNA in head and neck squamous cell carcinoma (HNSCC) cell lines led to an increase in G0 phase. Although, to establish a quiescent tumor cell model, we utilized Tet-on shRNA to regulate CYLD expression, the knockdown efficacy has been insufficient. Therefore, further investigation is needed.
Besides, we established a dormant bone marrow-disseminated tumor cell (BM-DTC)-derived subclone using OSCC cell line, HEp3. Using this subclone, we reported that intrinsic TGF-β2-SDF-1-CXCR4 signaling was important for slow-cycling state and drug resistance in dormant BM-DTC.
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