| Project/Area Number |
26861748
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Asahi University (2015-2016)
Meikai University (2014) |
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Principal Investigator |
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| Research Collaborator |
OHKOSHI Emika 青森大学, 薬学部, 准教授
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | がん幹細胞 / 口腔扁平上皮癌 / 化学療法 / 口腔がん / 薬剤耐性 |
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| Outline of Final Research Achievements |
We aimed to master treatment resistant cancer stem cells in oral squamous cell carcinoma. Firstly, we developed cisplatin-resistant oral squamous cell carcinoma cells that were maintained with media including cisplatin chemo reagent. In the results, the cisplatin-resistant oral squamous cell carcinoma cells held resistance characteristics not only to cisplatin but also to 5FU, doxorubicin, and other chemo reagents. Furthermore, when we analyzed characteristics of cancer stem-like cells in the multidrug-resistant oral squamous cell carcinoma cell, expression levels of CD44-which is known as a marker of cancer stem cells-were increased. Furthermore, expression levels of ABCG2, which is known as an ABC transporter, were also increased, and the function of drug ejection was elevated. These results indicated there are correlations between the acquisition of multi-drug resistance, the function of drug ejection, and cancer stem marker CD44.
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