Functional salivary gland regeneration using dedifferentiated fat cells

Research Project

Project/Area Number	26861759
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Surgical dentistry
Research Institution	Osaka Dental University
Principal Investigator	KISHIMOTO Naotaka 大阪歯科大学, 歯学部, 講師 (50610911)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000) Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords	再生医療 / 組織工学 / 脱分化脂肪細胞 / 幹細胞 / 唾液腺再生 / 細胞移植
Outline of Final Research Achievements	A human buccal fat pad (BFP) was obtained from a patient, who underwent oral and maxillofacial surgery. BFP was dissociated in a collagenase solution. We isolated dedifferentiated fat (DFAT) cells using the "ceiling culture method". This method used the adipocytes' characteristic that they have buoyancy. DFAT cells were passaged and used for flow cytometric analysis. DFAT cells were positive for Stro-1, CD44, CD90, CD105, CD166, and negative for CD19, CD45, CD106, CD146. We also obtained DFAT cells from GFP transgenic mice using the "ceiling culture method". The form of DFAT cells derived from GFP transgenic mice were evaluated using a fluorescence microscope.

Report

(3 results)