Elucidation of the bone microenvironment inducing EMT
| Project/Area Number |
26861761
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 骨簿小環境 / TGF-β / 細胞外基質 / EMT / 骨微小環境 / ＴＧＦ－β |
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| Outline of Final Research Achievements |
The aim of this study was to determine the effects of TGF-β and matrix stiffness on morphological changes and differentiation of osteoclast precursors (OCPs) using the pre-osteoclastic RAW264.7 cells. RAW264.7 cells treated with TGF-β1 and RANKL on stiff matrix showed morphological changes from round to spindle or polygonal morphology. Osteoclast differentiation was significantly induced by treating RAW264.7 cells with TGF-β1 and RAKL on stiff matrix. Otherwise, there is no osteoclast differentiation on soft matrix. There is a systematic relationship between tissue mechanics and differentiation.
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Report
(5 results)
Research Products
(6 results)
