Evaluation of induction method for dental pulp stem cells using PDGF
Project/Area Number |
26861766
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Orthodontics/Pediatric dentistry
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Research Institution | Tohoku University |
Principal Investigator |
Sugawara Yu 東北大学, 歯学研究科, 助教 (00636954)
|
Project Period (FY) |
2014-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | 歯髄幹細胞 / オリゴデンドロサイト / PDGF / 再生 / 歯 / BMP |
Outline of Final Research Achievements |
PDGF-AA promotes dental pulp stem cell differentiation to odontoblast, whereas PDGF-BB promotes dental pulp cell differentiation to oligodendrocyte and proliferation which might be regulated by ERK1/2 and Akt pathway via PDGFRb. And furthermore, PDGF-BB inhibits dental pulp cells differentiation to odontoblast which BMP2 promotes by inhibiting smad5 phosphorylation without inhibition of the expression of BMP2 receptors. From these results, because of the difference between differentiation markers mRNA expressions induced by PDGF-AA and PDGF-BB, it was showed PDGF-BB dose not take part on dental pulp cell differentiation to odontoblast but take part in dental pulp cell differentiation oligodendrocyte differentiation. Because these results are same when we used primary cultures mouse dental pulp stem cells, it was showed it is useful using cell line.
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Report
(5 results)
Research Products
(2 results)