The development of peptide which neutralizes macrophage colony-stimulating factor for inhibition of osteoclastogenesis.

• Project/Area Number: 26861769
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: KIMURA KEISUKE 東北大学, 歯学研究科(研究院), 助教 (70712909)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 破骨細胞 / M-CSF / ペプタイド / M-CFSペプタイド

Outline of Final Research Achievements

The recombinant c-fms proteins were inhibited osteoclastogenesis. We used various concentrations of recombinant c-fms proteins (0ng/ml,10ng/ml,100ng/ml,1000ng/ml,10000ng/ml). The effect of inhibition of osteoclastogenesis was dependent on the concentration of recombinant c-fms proteins

Research Products

• [Journal Article] IL-37 inhibits lipopolysaccharide-induced osteoclast formation and bone resorption in vivo (2016)
  • Author(s): Saeed, J., Kitaura, H., Kimura, K., Ishida, M., Sugisawa, H., Ochi, Y., Kishikawa, A., Takano-Yamamoto T
  • Journal Title: Immunology Letters Volume: 印刷中
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Muramyl dipeptide enhances lipopolysaccharide-induced osteoclast formation and bone resorption through increased RANKL expression in stromal cells. (2015)
  • Author(s): Ishida M, Kitaura H, Kimura K, Sugisawa H, Aonuma T, Takada H, Takano-Yamamoto T.
  • Journal Title: J Immunol Research Volume: 85 Pages: 132765-132765
  • DOI: 10.1155/2015/132765
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Intra-articular administration of an antibody against CFS-1 receptor reduces pain-related behaviors and inflammation in CFA-induced knee arthritis. (2015)
  • Author(s): lvarado-Vazquez PA., Morado-Urbina CE., Castaneda-Corral G., Acosta-Gonzalez RI., Kitaura, H., Kimura, K., Takano-Yamamoto T., Jimanez-Andrade JM
  • Journal Title: Neurosci. Lett. Volume: 584 Pages: 39-44
  • DOI: 10.1016/j.neulet.2014.09.053
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of Interleukin-4 on orthodontic tooth movement and associated root resorption. (2015)
  • Author(s): Hakami ZW., Kitaura, H., Kimura, K., Ishida, M., Sugisawa, H., Ida, H., Takano-Yamamoto T
  • Journal Title: Eur J Orthod. Volume: 37 Issue: 1 Pages: 87-94
  • DOI: 10.1093/ejo/cju016
  • Peer Reviewed / Open Access
• [Journal Article] An anti-c-Fms antibody inhibits osteoclastogenesis in a mouse periodontitis model (2014)
  • Author(s): Kimura K, Kitaura H, Fujii T, Ishida M, Hakami Z, Takano-Yamamoto T
  • Journal Title: Oral Diseases. Volume: 20(3) (in press) Issue: 3 Pages: 319-24
  • DOI: 10.1111/odi.12117
  • Peer Reviewed
• [Journal Article] Effect of cytokines on osteoclast formation and bone resorption during mechanical force loading of the periodontal membrane (2014)
  • Author(s): Kitaura H, Kimura K, Ishida M, Sugisawa H, Kohara H, Yoshimatsu M, Takano-Yamamoto T
  • Journal Title: Scientific World Journal Volume: 19 Pages: 1-7
  • DOI: 10.1155/2014/617032
  • Peer Reviewed / Open Access
• [Journal Article] he role of Th1 cytokines on mechanical loading-induced osteoclastogenesis and bone resorption. (2014)
  • Author(s): Kitaura, H., Kimura, K., Ishida, M., Hakami Z., Sugisawa, H., Saeed J., Kohara H., Yoshimatsu M., Takano-Yamamoto T
  • Journal Title: Interface Oral Health Science 2014 Volume: 1 Pages: 269-279
  • Open Access
• [Journal Article] Effect of macrophage colony-stimulating factor receptor c-Fms antibody on lipopolysaccharide-induced pathological osteoclastogenesis and bone resorption. (2014)
  • Author(s): Kimura, K., Kitaura, H., Ishida, M., Hakami Z., Saeed J., Sugisawa, H., Takano-Yamamoto T
  • Journal Title: Interface Oral Health Science 2014. Volume: 1 Pages: 259-267
  • Open Access
• [Presentation] 歯根膜圧迫による破骨細胞形成に対するIL-18の影響およびIL-12との相互作用 (2015)
  • Author(s): 越智由美子、北浦英樹、木村桂介、石田匡彦、杉澤晴紀、ジャファリサイード、岸川明子、山本照子
  • Organizer: 第66回東北大学歯学会
  • Place of Presentation: 宮城
  • Year and Date: 2015-12-12
• [Presentation] 矯正学的歯の移動における破骨細胞形成に関して骨髄移植を利用したキメラマウスによる解析 (2015)
  • Author(s): 北浦英樹、木村桂介、石田匡彦、杉澤晴紀、越智由美子、ジャファリ サイード、岸川明子、山本照子
  • Organizer: 第74回日本矯正歯科学会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-18
• [Presentation] 先天性疾患を伴う矯正歯科患者に対する生活の質のアンケート調査 (2015)
  • Author(s): 木村桂介、北浦英樹、黒木毅、石田匡彦、佐久間知子、長谷川博、山本照子
  • Organizer: 第74回日本矯正歯科学会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-18
• [Presentation] 矯正学的歯の移動による破骨細胞形成に対するIL-18の影響およびIL-12との相互作用の検討 (2015)
  • Author(s): 越智由美子、北浦英樹、木村桂介、石田匡彦、杉澤晴紀、ジャファリサイード、岸川明子、山本照子
  • Organizer: 第74回日本矯正歯科学会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-18
• [Presentation] TiNコーティングを施した歯科矯正用ワイヤーの耐食性および機械的特性の検討 (2015)
  • Author(s): 杉澤晴紀、北浦英樹、上田恭介、木村桂介、石田匡彦、越智由美子、岸川明子、山本照子
  • Organizer: 第74回日本矯正歯科学会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-18
• [Presentation] 顎顔面非対称の認識を評価する質問票の信頼性および妥当性の検討 (2015)
  • Author(s): 黒木毅、北浦英樹、木村桂介、石田匡彦、小林考敬、小原紀明、出口徹、山本照子
  • Organizer: 第74回日本矯正歯科学会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-18
• [Presentation] Analysis of contribution of marrow stromal cells and bone marrow macrophages to mechanical loading-induced osteoclastogenesis and bone resorption (2015)
  • Author(s): Kitaura H, Kimura K, Ishida M, Sugisawa H, Ochi Y, Saeed J, Kishikawa A, Takano-Yamamoto T
  • Organizer: The 37th Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: アメリカ シアトル
  • Year and Date: 2015-10-09
  • Int'l Joint Research
• [Presentation] Effect of IL-18 on mechanical loading-induced osteoclastogenesis and bone resorption solely, and in synergy with IL-12 (2015)
  • Author(s): Ochi Y, Kitaura H, Kimura K, Ishida M, Sugisawa H, Saeed J, Kishikawa A, Takano-Yamamoto T
  • Organizer: The 37th Meeting of the American Society for Bone and Mineral Research. Seatle, U.S.A. Oct 9th-12th 2015.
  • Place of Presentation: アメリカ シアトル
  • Year and Date: 2015-10-09
  • Int'l Joint Research
• [Presentation] メカニカルストレスによる破骨細胞形成および骨吸収に対するIL-18の影響の解析 (2015)
  • Author(s): 越智由美子、北浦英樹、木村桂介、石田匡彦、杉澤晴樹、岸川明子、山本照子
  • Organizer: 57回歯科基礎医学会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-11
• [Presentation] Analysis of Ni ion release and mechanical properties of orthodontic Ni-Ti wire after ion plating with TiN. (2015)
  • Author(s): Sugisawa H., Kitaura H., Ueda K., Kimura K., Ishida M., Saeed J., Ochi Y, Kishikawa A., Takano-Yamamoto T
  • Organizer: Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminar 2015
  • Place of Presentation: 宮城 蔵王
  • Year and Date: 2015-08-04
  • Int'l Joint Research
• [Presentation] LPSが誘導する病的破骨細胞形成および骨吸収に対するMDP（muramyl dipeptide）の作用の検討 (2015)
  • Author(s): 石田匡彦、北浦英樹、木村桂介、杉澤晴紀、山本照子
  • Organizer: 第1回日本骨免疫学会
  • Place of Presentation: 宮古島
  • Year and Date: 2015-06-30
• [Presentation] 口唇裂口蓋裂などの先天疾患を伴う矯正患者に対する顎口腔機能に関する問診票による調査 (2015)
  • Author(s): 木村桂介、北浦英樹、黒木毅、石田匡彦、佐久間知子、遠藤学、長谷川博、山本照子
  • Organizer: 第39回日本口蓋裂学会
  • Place of Presentation: 東京
  • Year and Date: 2015-05-21
• [Presentation] Analysis of effect of IL-4 on mechanical loading-induced osteoclast formation and bone resorption. (2014)
  • Author(s): Kitaura H, Hakami Z., Kimura K, Ishida M, Sugisawa H, Takano-Yamamoto T.
  • Organizer: The 62nd Annual Meeting of Japanese Association for Dental Research
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2014-12-04 – 2014-12-05
• [Presentation] Effect of muramyl dipeptide on LPS-induced osteoclast formation and bone resorption. (2014)
  • Author(s): Ishida M, Kitaura H, Kimura K, Sugisawa H, Aonuma T, Takada H, Takano-Yamamoto T
  • Organizer: The 62nd Annual Meeting of Japanese Association for Dental Research
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2014-12-04 – 2014-12-05
• [Presentation] Analysis of the corrosion resistance of titanium nitride coating on orthodontic wires. (2014)
  • Author(s): Sugisawa H., Kitaura H., Ueda K., Kimura K., Ishida M., Takano-Yamamoto T.
  • Organizer: The 62nd Annual Meeting of Japanese Association for Dental Research
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2014-12-04 – 2014-12-05
• [Presentation] 矯正学的歯の移動におけるIL-4の効果 (2014)
  • Author(s): 北浦英樹、ザキウェリハカミ、木村桂介、石田匡彦、杉澤晴紀、井田裕人、ジャファリサイード、山本照子
  • Organizer: 第73回日本矯正歯科学会
  • Place of Presentation: 千葉
  • Year and Date: 2014-10-20 – 2014-10-22
• [Presentation] LPSが引き起こす破骨細胞形成および骨吸収に対するMDPの作用の検討 (2014)
  • Author(s): 石田匡彦、北浦英樹、木村桂介、ザキウェリハカミ、杉澤晴紀、ジャファリサイード、高田春比古、山本照子
  • Organizer: 第73回日本矯正歯科学会
  • Place of Presentation: 千葉
  • Year and Date: 2014-10-20 – 2014-10-22
• [Presentation] ffect of IL-4 on mechanical loading-induced osteoclastogenesis and bone resorption (2014)
  • Author(s): Kitaura H, Hakami Z., Kimura K, Ishida M, Saeed J, Sugisawa H, Takano-Yamamoto T
  • Organizer: The 36th Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Houston, U.S.A
  • Year and Date: 2014-09-12 – 2014-09-15
• [Presentation] Muramyl dipeptide enhances Lipopolysaccharide-induced osteoclast formation and bone resorption by enhance of RANKL expression. (2014)
  • Author(s): Ishida M, Kitaura H, Kimura K, Saeed J, Sugisawa H, Takada H., Takano-Yamamoto T
  • Organizer: The 36th Meeting of the American Society for Bone and Mineral Research.
  • Place of Presentation: Houston, U.S.A
  • Year and Date: 2014-09-12 – 2014-09-15
• [Presentation] The corrosion resistance of Titanium Nitride (TiN) plating on an orthodontic wires (2014)
  • Author(s): Sugisawa H., Kitaura H., Ueda K., Kimura K., Ishida M., Saeed J., Ochi Y, Takano-Yamamoto T.
  • Organizer: 9th International Workshop on Biomaterials in Interface Science. Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminar 2014
  • Place of Presentation: Miyagi, Japan
  • Year and Date: 2014-08-26 – 2014-08-27
• [Presentation] LPS誘導病的骨吸収に対するMDPの作用の検討 (2014)
  • Author(s): 石田匡彦、北浦英樹、木村桂介、杉澤晴紀、山本照子
  • Organizer: 第32回日本骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 2014-07-24 – 2014-07-26
• [Presentation] Macrophage colony-stimulating factor中和による機械的荷重により誘導される破骨細胞形成および破歯細胞形成に対する抑制効果 (2014)
  • Author(s): 北浦英樹、木村桂介、石田匡彦、ハカミ ザキ、サイード ジャファリ、杉澤 晴紀、小原悠、吉松昌子、山本照子
  • Organizer: 第53回日本生体医工学会
  • Place of Presentation: 仙台
  • Year and Date: 2014-06-24 – 2014-06-26