Establishment of a differentiation method of periodontal ligament cells from human iPS cells, and development of dental implant with periodontal ligament.

• Project/Area Number: 26861770
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: KATO RYUSHI 東北大学, 歯学研究科(研究院), 助教 (90706699)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: iPS細胞 / 歯根膜 / 多分化能 / 歯根膜繊維 / 間葉系幹細胞

Outline of Final Research Achievements

The purpose of this study is establishment of a differentiation method of periodontal ligament (PDL) cells from iPS cells, which is generated by transfection of reprogramming genes in human PDL cells.
We first isolated human PDL cells. The PDL cells were transfected with plasmids for iPS cell generation. Three weeks after transfection, iPS cell-like colonies with clear edge were observed. In addition, we examined the experimental conditions with the aim of differentiation of the iPS cells into PDL cells.