The establishment of a novel isolation method of ameloblast and search of specific gene expression

• Project/Area Number: 26861784
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Okayama University
• Principal Investigator: Murakami Takashi 岡山大学, 大学病院, 講師 (00534786)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: エナメル芽細胞

Outline of Final Research Achievements

It has been impossible to reproduce tooth enamel when it was destroyed by caries. The standard treatment for a cavity is to fill the tooth by using restorative materials. Therefore, it has been expected that the elucidation of the differentiation and maturity of ameloblast to form enamel, and the mechanism to produce the substrate. However, it has been difficult to gather ameloblast from the dental germ in the developmental process. This study investigated a new technique to gather ameloblast effectively from a dental germ.