Toward the cell therapy for central nerve system disease with stem cells from human exfoliated deciduous teeth

• Project/Area Number: 26861791
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: KITAMURA Takamasa 徳島大学, 大学院医歯薬学研究部, 助教 (50614020)
• Research Collaborator: SUGIMOTO Asuna 徳島大学, 大学院医歯薬学研究部, 大学院生
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 圧迫培養 / 含歯性嚢胞 / 歯原性上皮細胞 / 歯肉線維芽細胞 / 骨芽細胞 / 骨吸収 / 圧迫培養法 / 骨形成

Outline of Final Research Achievements

I got the following research result with a main study.
Molecular mechanism of bone resorption by a dentigerous cyst is unknown, but it has been suspected that it makes bone resorption to press to the surrounding organization by the increased pressure in the cyst. It was considered using the press cultivation which assumed odontogenic cysts what kind of influence the cell external pressure power had on the cell of which a dentigerous cyst is composed. Since when molecular group related to a bone desorption was analyzed using modal Real time RT-PCR, cytokines related to a bone resorption were released from a mesenchymal tissue to continuous low pressure, and putting it in the osteoblast, in fact, the mount of mRNA expression that related to a bone formation increased. From the above, it was suggested that for the gentle and quiet bone resorption due to the cyst, a mesenchymal tissue plays a central role in a bone resorption.

Research Products

• [Journal Article] Recruitment of mesenchymal stem cells by stromal cell-derived factor 1 α in pulp cells from deciduous teeth. (2015)
  • Author(s): Yuki Akazawa, Tomokazu Hasegawa, Yoshitaka Yoshimura, Naoyuki Chosa, Takeyoshi Asakawa, Kimiko Ueda, Asuna Sugimoto, Takamasa Kitamura, Hiroshi Nakagawa, Akira Ishisaki, and Tsutomu Iwamoto
  • Journal Title: Int J Mol Med Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Dental findings and management in a child with hypomelanosis of Ito. (2014)
  • Author(s): Tomokazu Hasegawa, Yuki Akazawa, Takamasa Kitamura, Asuna Sugimoto, Kimiko Ueda, Tsutomu Iwamoto
  • Journal Title: Pediatric Dental Journal Volume: 24 Issue: 3 Pages: 173-177
  • DOI: 10.1016/j.pdj.2014.08.002
  • Peer Reviewed / Open Access
• [Journal Article] 幼児期の小児の口蓋に発生した血管腫の１例 (2014)
  • Author(s): 長谷川智一, 赤澤友基, 永井宏和, 北村尚正, 石丸直澄, 上田公子, 中川 弘, 郡 由紀子, 山本愛美, 岩本 勉
  • Journal Title: 小児歯科学雑誌 Volume: 3 Pages: 448-458
  • NAID: 130005084435
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The role of extracellular ATP-mediated purinergic signaling in bone, cartilage, and tooth tissue (2014)
  • Author(s): Tsutomu Iwamoto, Asuna Sugimoto, Takamasa Kitamura, Yuki Akazawa, Tomokazu Hasegawa
  • Journal Title: J Oral Biosci Volume: 56 Issue: 4 Pages: 131-135
  • DOI: 10.1016/j.job.2014.07.003
  • Peer Reviewed / Open Access
• [Presentation] 乳歯歯根膜由来細胞のSDF-1αを介した歯周組織恒常性に関わる細胞間相互作用 (2015)
  • Author(s): 長谷川智一, 赤澤友基, 吉村善隆, 帖佐直幸, 浅川剛吉, 杉本明日菜, 北村尚正, 上田公子, 中川 弘, 白石真紀, 石崎 明, 岩本 勉
  • Organizer: 第53回小児歯科学会大会
  • Place of Presentation: 広島国際会議場（広島県中区）
  • Year and Date: 2015-05-21 – 2015-05-22
• [Presentation] p-HPPHを分子標的としたフェニトインによる歯肉増殖症に対する新規治療薬の開発 (2014)
  • Author(s): 中川弘, 山本愛美, 赤澤友基, 北村尚正, 長谷川智一, 岩本勉
  • Organizer: 第31回日本障害者歯科学会総会および学術大会
  • Place of Presentation: 仙台国際センター（仙台市青葉区）
  • Year and Date: 2014-11-14 – 2014-11-16
• [Presentation] 口腔保健センターにおける青少年期患者の定期口腔管理の実態と問題点 (2014)
  • Author(s): 森本みどり, 石川英子, 岡本悦子, 河野恵, 坂東弘子, 森重代, 横井久美子, 郡由紀子, 上田公子, 尼寺理恵, 山本愛美, 北村尚正, 中川弘, 原田桂子
  • Organizer: 第31回日本障害者歯科学会総会および学術大会
  • Place of Presentation: 仙台国際センター（仙台市青葉区）
  • Year and Date: 2014-11-14 – 2014-11-16
• [Presentation] 含歯性嚢胞の間葉系結合組織層が骨吸収に与える影響 (2014)
  • Author(s): 北村尚正, 杉本明日菜, 中川 弘, 赤澤友基, 長谷川智一, 岩本 勉
  • Organizer: 第56回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2014-09-26 – 2014-09-27
• [Presentation] 幼児期の小児の口蓋に発生した血管腫の１例 (2014)
  • Author(s): 長谷川智一, 赤澤友基, 北村尚正, 上田公子, 中川 弘, 岩本 勉
  • Organizer: 第52回小児歯科学会大会
  • Place of Presentation: きゅりあん（東京都品川区）
  • Year and Date: 2014-05-16 – 2014-05-17
• [Presentation] 当科にて長期的口腔内管理を行った伊藤白斑の1例 (2014)
  • Author(s): 赤澤友基, 長谷川智一, 久保藤原百合, 北村尚正, 上田公子, 中川 弘, 岩本 勉
  • Organizer: 第52回小児歯科学会大会
  • Place of Presentation: きゅりあん（東京都品川区）
  • Year and Date: 2014-05-16 – 2014-05-17
• [Presentation] 鼻腔底に近接した上顎正中埋伏過剰歯由来の含歯性嚢胞の1例 (2014)
  • Author(s): 北村尚正, 郡由紀子, 赤澤友基, 長谷川智一, 岩本 勉
  • Organizer: 第52回小児歯科学会大会
  • Place of Presentation: きゅりあん（東京都品川区）
  • Year and Date: 2014-05-16 – 2014-05-17