| Project/Area Number |
26861792
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
Hoshi Kenji 九州大学, 大学病院, 助教 (90569964)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 軟骨細胞 / メカニカルストレス / microRNA / micro RNA / 下顎頭軟骨 / 間葉系幹細胞 / 機械的刺激 / マイクロRNA |
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| Outline of Final Research Achievements |
We examined the chondrogenesis of mesenchymal stem cells under mechanical stress, focusing on micro RNAs (miRNA). Mesenchymal stem cells derived from fetal mouse condylar cartilages were seeded on a culture device that can apply tensile stress. We loaded tensile stress for 3 hours, 1 day and 2 days. After the application of tensile stress, microarray analysis was performed to detect miRNAs whose expression levels were characteristically changed. We also examined whether the detected miRNAs target chondrogenic marker genes. According to this analysis, the expression levels of miR-134-5p, 152-3p, 29a-3p, 431-5p, 145a-5p, 342-3p and 125a-3p were increased in loaded groups compared with control groups. These miRNAs were found to target Col2a or Sox9.
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