Elucidation of the molecular regulation mechanism of injury and recovery of periodontal tissue by orthodontic treatment

• Project/Area Number: 26861804
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: Muraoka Rina 松本歯科大学, 歯学部, 助教 (20549430)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 歯周組織 / 歯根膜 / メカニカルストレス / 歯科矯正 / 修復 / リモデリング / 歯科矯正学 / 細胞分化 / 組織修復

Outline of Final Research Achievements

The purpose of the study was to determine the cell dynamics in periodontal ligament in response to mechanical stress during orthodontic movement. Mechanical stress during orthodontic movement promoted the increase in the number of cells in the periodontal ligament on both tension and pressure sides. The increase in the number of cells in the periodontal ligament is believed to be due to the migration and cell division of undifferentiated mesenchymal cells.
Also HSP47 is expressed in PDL fibroblasts on the compressed side damaged by application of mechanical stress and contributes to the repair of collagen tissue by activating PDL fibroblasts, supporting recovery from cell damage. HSP47 investigated in this study acts differently depending of the time of expression.

Research Products

• [Journal Article] HSP47 as a Possible Molecular Chaperone for the collagen synthesis in the Mouse Periodontal Ligament Cells due to Orthodontic Force (2017)
  • Author(s): Muraoka R, Nakano K, Yamada K, Kawakami T
  • Journal Title: Int J Dentistry Oral Sci. Volume: ４（1） Pages: 387-394
  • DOI: 10.19070/2377-8075-1700078
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Functional Role of HSP47 in the Periodontal Ligament Subjected to Occlusal Overload in Mice. (2015)
  • Author(s): Hiroaki Mimura, Tatsuo Takaya, Saeka Matsuda, Keisuke Nakano, Rina Muraoka, Mihoko Tomida, Norimasa Okafuji, Takeo Fujii, Toshiyuki Kawakami.
  • Journal Title: Int J Med Sci Volume: 13 Issue: 4 Pages: 248-254
  • DOI: 10.7150/ijms.14129
  • NAID: 120006242001
  • Peer Reviewed / Open Access
• [Journal Article] Histological Evaluation of Periodontal Ligament in Response to Orthodontic Mechanical Stress in Mice (2015)
  • Author(s): Kaneko K, Matsuda S, Muraok R, Nakano K, Iwasaki T, Tomida M, Tsujigiwa H, Nagatsuka H, and Kawakami T
  • Journal Title: Int J Med Sci Volume: 12 Issue: 9 Pages: 689-694
  • DOI: 10.7150/ijms.12883
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 歯科矯正学的メカニカルストレスが引き起こすマウス歯根膜におけるHSP70の免疫組織化学的発現推移 (2016)
  • Author(s): 村岡理奈、中野敬介、川上敏行、山田一尋
  • Organizer: 第75回 日本矯正歯科学会大会
  • Place of Presentation: アスティとくしま，徳島,市，徳島県
  • Year and Date: 2016-11-09
• [Presentation] HSP70 in recovery of the mouse PDL by mechanical stress (2016)
  • Author(s): Muraoka R, Nakano K, Yamada K, Kawakami T
  • Organizer: 13th Biennial Congress of European Association of Oral Medicine (EAOM) Incorporating the World Workshop on Oral Medicine
  • Place of Presentation: Torino, Italy
  • Year and Date: 2016-09-15
  • Int'l Joint Research
• [Presentation] メカニカルストレスが惹起するマウス歯根膜の傷害と回復におけるHSP70の発現推移 (2016)
  • Author(s): 村岡理奈、中野敬介、川上敏行、山田一尋
  • Organizer: 第59回 日本口腔科学会中部地方部会
  • Place of Presentation: 松本歯科大学，塩尻市，長野県
  • Year and Date: 2016-09-11
• [Presentation] 歯科矯正学的メカニカストレスによる歯周組織の改造 (2016)
  • Author(s): 金子圭子、松田紗衣佳、村岡理奈、中野敬介、富田美穂子、辻極秀次、長塚 仁、川上敏行
  • Organizer: 第59回 日本口腔科学会中部地方部会
  • Place of Presentation: 松本歯科大学，塩尻市，長野県
  • Year and Date: 2016-09-11
• [Presentation] HSP70 expression as recovery reaction in the mouse periodontal tissues (2016)
  • Author(s): Muraoka R, Nakano K, Matsuda H, Kurata K, Yamada K, Kawakami T
  • Organizer: The 2016 AADR/CADR Annual Meeting & Exhibition
  • Place of Presentation: Los Angeles Convention Center（U.S.A., Los Angeles）
  • Year and Date: 2016-03-18
• [Presentation] 歯科矯正力が惹起するマウス歯根膜細胞におけるHSP47の免疫組織化学的発現推移 (2015)
  • Author(s): 村岡理奈，倉田和之、松田浩和、中野敬介、川上敏行、山田一尋
  • Organizer: 第74回日本矯正歯科学会大会
  • Place of Presentation: マリンメッセ福岡(福岡県)
  • Year and Date: 2015-11-19
• [Presentation] HSP27 expression as a possible molecular chaperone in the periodontal ligament cells due to orthodontic mechanical stress. (2015)
  • Author(s): Muraoka R, Kurata K, Nakano K, Yamada K, Kawakami T
  • Organizer: The 46th International Congress of the Italian Society of Orthodontics
  • Place of Presentation: The Congress Venue（Itary, Milano）
  • Year and Date: 2015-10-30
  • Int'l Joint Research
• [Presentation] メカニカルストレスが惹起するマウス歯根膜におけるHSP70の免疫組織化学的発現推移 (2015)
  • Author(s): 村岡理奈，松田浩和，山田一尋，中野敬介，川上敏行
  • Organizer: 第24回硬組織再生生物学会学術大会・総会
  • Place of Presentation: 大阪歯科大学創立 100 周年記念館(大阪府)
  • Year and Date: 2015-08-22
• [Presentation] 歯科矯正力により惹起するマウス歯周組織改造現象における細胞動態 (2014)
  • Author(s): 村岡理奈，金子圭子，中野敬介，山田一尋，川上敏行
  • Organizer: 第73回日本矯正歯科学会大会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2014-10-22
• [Presentation] Periodontal Tissue Remodeling due to Orthodontic Mechanical Stress. (2014)
  • Author(s): Muraoka R, Kaneko K, Nakano K, Yamada K and Kawakami T
  • Organizer: The 7th Asian Science Seminar & 第23回硬組織再生生物学会学術大会・総会
  • Place of Presentation: 中山医学大学口腔医学院（台中，台湾）
  • Year and Date: 2014-08-21
• [Book] Chapter 9. Injury and recovery of the periodontal ligament: from a view of point of developmental biology. In Advances in Medicine and Biology, Vol 111. Berhardt LV ed. (2017)
  • Author(s): Kawakami T, Tsujigiwa H, Takaya T, Kaneko K, Mimura H, Matsuda S, Muraoka R, Tomida M, Okafuji N, Fujii T, Nakano K and Nagatsuka H.
  • Publisher: Nova Biomedical Publisher, NY, USA