Changes in biomarkers after initial periodontal treatment in gingival crevicular fluid from patients with chronic periodontitis presenting with drug-induced gingival overgrowth

Research Project

Project/Area Number	26861809
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Periodontology
Research Institution	Niigata University
Principal Investigator	SHIMIZU TARO 新潟大学, 医歯学総合病院, 医員 (60609121)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000) Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	歯肉増殖症 / 歯肉溝浸出液 / マルチプレックスサスペンション / 薬剤性歯肉増殖症 / マルチプレックスサスペンションシステム
Outline of Final Research Achievements	The aim of the present study was to simultaneously quantify the levels of 14 biomarkers in gingival crevicular fluid (GCF) samples from sites with both gingival overgrowth (GO) and chronic periodontitis (CP) by multiplex immunoassays.In addition, we examined the relationship between the subgingival microbiota and GCF biomarkers before and after therapy. we used a multiplex bead immunoassay to detect levels of 12 biomarkers including IL-1β and TGF-β1 in GCF from GO, and we also clarified the presence and quantity of T. denticola from the same sites.