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Application to periodontal tissue regeneration therapy of local blood flow improving agent

Research Project

Project/Area Number 26861811
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Periodontology
Research InstitutionOsaka University

Principal Investigator

MIKI KOJI  大阪大学, 歯学部附属病院, 医員 (10598395)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords歯周組織再生 / 歯周再生医学 / 塩化カルプロニウム / ムスカリン受容体
Outline of Final Research Achievements

Methods:Expression of cholinergic component in human periodontal ligament cells (HPDL) was examined by RT-PCR and immunohistochemistry. And topical application of carpronium chloride on rat calvarial defect was observed whether to promote new bone formation. Results: All of the mAchR mRNA were expressed in HPDL. Choline acetyl transferase (ChAT), Vesicular acetylcholine transporter (VAChT), Acetylcholine esterase (AChE) and various nAChR mRNA were detected in HPDL. Expression of ChAT, VAChT and AChE in HPDL was detected in immunohistochemistry. And Topical application of carpronium chloride on rat calvarial defect promoted new bone formation. Conclusion: Periodontal ligament cells have various necessary cholinergic components such as ACh synthesis, reception, degradation. These results suggest that non-neuronal cholinergic system in periodontal ligament cells and carpronium chloride may be involved in the homeostasis, wound healing and regeneration of periodontal tissues.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2015 2014

All Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] Expression of non-neuronal cholinergic system in periodontal ligament cells2015

    • Author(s)
      Koji MIKI, Motozo YAMASHITA, Manabu YANAGITA, Kenta MORI, Masahiro KITAMURA and Shinya MURAKAMI
    • Organizer
      第63回JADR学術大会
    • Place of Presentation
      福岡県福岡市 福岡国際会議場
    • Year and Date
      2015-10-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 高齢双生児者における歯周病細菌抗体価と遺伝要因の解析2014

    • Author(s)
      東山弥生、山下元三、三木康史、池上久仁子、津島賢一郎、川口佳保里、久保田実木子、小河大、西原千尋、藤原健太、池永友里、森永秦樹、北垣次郎太、柳田学、野崎剛徳、久留島悠子、榎木香織、松田謙一、池邉一典、北村正博、前田芳信、村上伸也
    • Organizer
      第141回秋季日本歯科保存学会学術大会
    • Place of Presentation
      山形
    • Year and Date
      2014-10-31
    • Related Report
      2014 Research-status Report
  • [Presentation] 初期固定力の不十分なインプラントに対するFGF-2の安定性獲得促進効果2014

    • Author(s)
      野崎剛徳、田中利江、沢田啓吾、小笹匡雄、三木康史、池上久仁子、阪下裕美、山本智美、宮内静香、北垣次郎太、山下元三、山田聡、白石紀子、安齋淳、北村正博、村上伸也
    • Organizer
      第57回秋季日本歯周病学会学術大会
    • Place of Presentation
      神戸
    • Year and Date
      2014-10-19
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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