| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Diabetes is often associated with increased prevalence and advanced pathology of periodontal disease. We hypothesized that gingival epithelial cells modify periodontal disease progression, and predicted that hyperglycemia would activate an inflammatory response in human gingival epithelial cells (HGECs). We tested this in immortalized HGECs isolated from periodontal tissue. The HGECs cultured in high and normal glucose (NG) conditions. The epi 4 cells showed increased inflammatory cytokines and toll-like receptor 2 protein secretion and mRNA expression when cultured in HG compared with NG. These effects were not associated with increased cell proliferation and were not observed in a hyperosmolar control group. The increased IL-8 secretion in HG was inhibited by pretreatment with an antioxidant and protein kinase C inhibitor. These suggest a potential mechanism for how, in diabetes, hyperglycemia could provoke an excessive host inflammatory response, exacerbating periodontal disease.
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