Role of new Cytokines IL-35 in inflammation bone metabolism

• Project/Area Number: 26861824
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontology
• Research Institution: Aichi Gakuin University
• Principal Investigator: Kamiya Yosuke 愛知学院大学, 歯学部, 助教 (70572808)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: IL-35 / サイトカイン / 骨代謝

Outline of Final Research Achievements

Osteoclast differentiation marker (MMP-9, Cathepsin K, TRAP) gene expression, osteoclast formation and osteoclast activity in RAW 264.7 cells were significantly increased by RANKL and IL-35 compared with RANKL alone. The phosphorylations of ERK and p-38 were increased by RANKL and IL-35 compared with RANKL or IL-35 alone. The number of osteoclast by RANKL and IL-35 were significantly inhibited by pretreatment with ERK inhibitor compared with no treatment. Therefore, the effect by IL-35 and RANKL promoted synergistic effect on osteoclast formation mainly via ERK signaling pathway.