| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
The purpose of this study was to investigate the preventive effects of topical application of catechins on tongue oxidative stress induced by 5-FU administration in rats. Rats were divided into four groups of seven rats each: a negative control group (saline + ointment without catechins), a positive control group (5-FU + ointment without catechins), and two experimental groups (5-FU + ointment containing 0.1% or 0.5% catechins). Topical application of each ointment to the ventral surface of the tongue was performed once a day for 5 days. The level of 8-OHdG was determined to evaluate oxidative stress.
The ratios of 8-OHdG positive cells were higher in the positive control group than in the negative control group. On the other hand, those in the 0.5% green tea catechin group, but not in the 0.1% green tea catechin group, were lower than the positive control group.
Topical application of ointment containing 0.5% catechins could prevent tongue oxidative stress in 5-FU administered rats.
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