Observation of early dental plaque formation process using the FISH method

• Project/Area Number: 26861831
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Social dentistry
• Research Institution: Kyushu University
• Principal Investigator: Tsuboi Hidenori 九州大学, 歯学研究科(研究院), 共同研究員 (60614253)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: デンタルプラーク / FISH

Outline of Final Research Achievements

To investigate the primary dental plaque formation, we constructed the fluorescence in situ hybridization (FISH) method that could observe the non-destructive plaque and classified the bacteria on hydroxyapatite disk. As a result, we discovered that some bacteria made the small colonies of mountain-like formation in early dental plaque and time passed, become fused the colonies made large dental plaque. Next, we mixed the certain bacteria and made similar colonies in vitro. We found that Neisseria genus and Actinomyces genus were essential for aggregation of bacteria and making the similar mountain-like colonies.