Development of surface modification techniques for coherent X-ray diffraction imaging of in-solution biological samples.
| Project/Area Number |
26870006
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Quantum beam science
Nanobioscience
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| Research Institution | Hokkaido University |
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Principal Investigator |
Kimura Takashi 北海道大学, 電子科学研究所, 助教 (50531472)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | X線光学 / X線イメージング / 超精密加工 / X線自由電子レーザー / コヒーレント回折イメージング / コヒーレントX線回折イメージング / X線顕微鏡 / コヒーレント光学 / 生体分子パターニング / 自己組織化単分子膜 / 電子ビームリソグラフィ / 環境セル / バイオイメージング / 単分子解析 |
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| Outline of Final Research Achievements |
In this research, I developed surface modification techniques in order to advance the potential of in-solution biological sample imaging by X-ray free-electron laser coherent diffraction microscopy. The surface modification technique we developed includes the fabrication of 30-nm-damenter dot pattern with electron beam lithography on 200-nm-thickness silicon nitride membrane which is used as sample holder for coherent diffraction microscopy. On each dot pattern, we successfully mounted protein molecules with the assistance of self-assembled monolayer. Coherent diffraction patterns from such uniquely fabricated samples were obtained at Japanese X-ray free-electron laser facility SACLA (SPring-8 Angstrom Compact free-electron LAser) and the image was reconstructed by using phase retrieval algorithms.
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Report
(3 results)
Research Products
(20 results)
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[Journal Article] Photoelectron Angular Distribution for Two-Photon Ionization of Helium by Ultrashort Extreme Ultraviolet Free Electron Laser Pulses2013
Author(s)
R. Ma, K. Motomura, K.L. Ishikawa, S. Mondal, H. Fukuzawa, A. Yamada, K. Ueda, K. Nagaya, S. Yase, Y. Mizogichi, M. Yao, A. Rouzée, A. Hundermark, M.J.J. Vrakking, P. Johnsson, M. Nagasono, K. Tono, T. Togashi, Y. Senba, H. Ohashi , M. Yabashi, and T. Ishikawa
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Journal Title
J. Phys. B: At. Mol. Opt. Phys.
Volume: 46
Issue: 16
Pages: 164018-164018
DOI
Related Report
Peer Reviewed
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