A high-resoluton map of SBP1 interactomes in Plasmodium falciparum-infected erythrocytes.

• Project/Area Number: 26870021
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology; Veterinary medical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: Takano Ryo 帯広畜産大学, 原虫病研究センター, 研究員 (90722588)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 熱帯熱マラリア / 病原性 / インタラクトーム / マウレル裂 / 輸送タンパク質 / 分泌タンパク質 / 重症マラリア / 免疫沈降 / マラリア / 赤血球 / 質量分析 / プロテオミクス解析 / ノックアウト原虫 / ショットガンプロテオーム

Outline of Final Research Achievements

Plasmodium falciparum parasites, a causative agent of human malaria, export numerous proteins to the host erythrocyte membrane and cytosol. These exported proteins, sometimes referred to as the exportome, facilitate host cell remodeling and are directly associated with the pathology and pathogenesis of severe malaria. Therefore, to understand the molecular basis of severe malaria, it is essential to clarify the exportome. Here, I conducted FLAG-tag-based immunoprecipitation with cell lysates of erythrocytes infected with parasites that expressed FLAG-tagged SBP1, a known Maurer's cleft component, and analyzed the precipitated proteins by using high-sensitive mass spectrometry. The precipitated proteins were predominantly host cytoskeleton and Maurer's cleft components. I further identified novel host and parasite proteins that were recruited to and localized in Maurer's clefts, suggesting the involvement of these proteins in the severe malaria caused by Plasmodium falciparum.

Research Products

• [Journal Article] Involvement of β-defensin 130 (DEFB130) in the macrophage microbicidal mechanisms for killing Plasmodium falciparum (2017)
  • Author(s): Terkawi MA, Takano R, Furukawa A, Murakoshi F, Kato K.
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 41772-41772
  • DOI: 10.1038/srep41772
  • NAID: 120006219999
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Characterization of a Toxoplasma gondii calcium calmodulin-dependent protein kinase homolog. (2016)
  • Author(s): Kato K, Sugi T, Takemae H, Takano R, Gong H, Ishiwa A, Horimoto T, Akashi H
  • Journal Title: Parasites & Vectors Volume: 9(1) Issue: 1 Pages: 405-405
  • DOI: 10.1186/s13071-016-1676-1
  • NAID: 120006390949
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Isolation and co-cultivation of human macrophages and neutrophils with Plasmodium falciparum-parasitized erythrocytes: An optimized system to study the phagocytic activity to malarial parasites (2016)
  • Author(s): Terkawi MA, Takano, R, Kato K.
  • Journal Title: Parasitology International Volume: In press Issue: 5 Pages: 30025-3
  • DOI: 10.1016/j.parint.2016.03.005
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Heparin interacts with elongation factor 1α of Cryptosporidium parvum and inhibits invasion. (2015)
  • Author(s): Inomata A, Murakoshi F, Ishiwa A, Takano R, et al.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 11599-11599
  • DOI: 10.1038/srep11599
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Influenza virus-host interactome screen as a platform for antiviral drug development. (2014)
  • Author(s): Watanabe T, Kawakami E, Shoemaker JE, Lopes TJ, Matsuoka Y, Tomita Y, Kozuka-Hata H, Gorai T, Kuwahara T, Takeda E, Nagata A, Takano R, Kiso M, Yamashita M, Sakai-Tagawa Y, Katsura H, Nonaka N, Fujii H, Fujii K, Sugita Y, Noda T, Goto H, Fukuyama S, Watanabe S, Neumann G, Oyama M, Kitano H, Kawaoka Y.
  • Journal Title: Cell Host and Microbe Volume: 16 Issue: 6 Pages: 795-805
  • DOI: 10.1016/j.chom.2014.11.002
  • Peer Reviewed / Open Access
• [Journal Article] Lambda-carrageenan treatment exacerbates the severity of cerebral malaria caused by Plasmodium berghei ANKA in BALB/c mice. (2014)
  • Author(s): Recuenco FC, Takano R, Chiba S, Sugi T, Takemae H, Murakoshi F, Ishiwa A, Inomata A, Horimoto T, Kobayashi Y, Horiuchi N, Kato K.
  • Journal Title: Malar J. Volume: 13 Issue: 1 Pages: 487-487
  • DOI: 10.1186/1475-2875-13-487
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] A high-resolution map of SBP1 interactomes in Plasmodium falciparum-infected erythrocytes. (2017)
  • Author(s): Ryo Takano et al.
  • Organizer: Japan-Brazil Malaria Research Workshop: fostering new partnerships
  • Place of Presentation: University of Sao Paulo (Sao Paulo, Brazil)
  • Year and Date: 2017-03-15
  • Int'l Joint Research / Invited
• [Presentation] A high-resolution map of SBP1 interactomes in Plasmodium falciparum-infected erythrocytes. (2016)
  • Author(s): Ryo Takano et al.
  • Organizer: The 15th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路夢舞台（兵庫県神戸市）
  • Year and Date: 2016-09-06
  • Int'l Joint Research
• [Presentation] Targeted proteomic dissection of Plasmodium falciparum Maurer's cleft compartments using SBP1. (2016)
  • Author(s): Ryo Takano et al.
  • Organizer: 第24回分子寄生虫学ワークショップ&第14回分子寄生虫・マラリア研究合同大会
  • Place of Presentation: 帯広畜産大学（北海道帯広市）
  • Year and Date: 2016-08-21
• [Presentation] マラリア原虫におけるNova-1の機能の探索 (2016)
  • Author(s): 野中基弘，高野量，加藤健太郎
  • Organizer: 第24回分子寄生虫学ワークショップ&第14回分子寄生虫・マラリア研究合同大会
  • Place of Presentation: 帯広畜産大学（北海道帯広市）
  • Year and Date: 2016-08-21
• [Presentation] マウレル裂プロテオミクスによる熱帯熱マラリア原虫赤血球寄生メカニズムの解析 (2016)
  • Author(s): 高野量，秦裕子，竹前等，尾山大明，加藤健太郎
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県・宮崎市）
  • Year and Date: 2016-03-19
• [Presentation] トキソプラズマのシスト形成に寄与する因子の探索 (2016)
  • Author(s): 村田優穂，高野量，五十嵐慎，加藤健太郎
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県・宮崎市）
  • Year and Date: 2016-03-19
• [Presentation] Differentiated gene profile of neutrophils induced by phagocytosis of Plasmodium falciparum-parasitized erythrocytes (2016)
  • Author(s): Mohamad Alaa Terkawi, Ryo Takano, Fumi Murakoshi, Kentaro Kato
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県・宮崎市）
  • Year and Date: 2016-03-19
• [Presentation] 熱帯熱マラリア原虫寄生赤血球にみられるマウレル裂を構成する因子の網羅的同定 (2015)
  • Author(s): 高野量，秦裕子，竹前等，尾山大明，加藤健太郎
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] 熱帯熱マラリア原虫分泌タンパク質の探索 (2014)
  • Author(s): 高野量，尾山大明，秦裕子，加藤健太郎
  • Organizer: 第22回分子寄生虫学ワークショップ＆第12回分子寄生虫・マラリア研究フォーラム合同大会
  • Place of Presentation: 帯広
  • Year and Date: 2014-08-31 – 2014-09-03
• [Book] 寄生虫学研究；材料と方法 2014年版，p.111-116 (2014)
  • Author(s): 高野量，Terkawi Mohamad Alaa，加藤健太郎
  • Total Pages: 179
  • Publisher: 三恵社
• [Book] 寄生虫学研究；材料と方法 2014年版，p.9-10 (2014)
  • Author(s): Terkawi Mohamad Alaa，高野量，加藤健太郎
  • Total Pages: 179
  • Publisher: 三恵社