Low-birth-weight neonate at Great earthquake disaster and genomic imprinting in placenta

• Project/Area Number: 26870032
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hygiene and public health; Obstetrics and gynecology
• Research Institution: Tohoku University
• Principal Investigator: Miyauchi Naoko 東北大学, 医学系研究科, 研究支援者 (60596162)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 産科学 / 母子保健 / エピゲノム / インプリンティング / 胎盤 / 低出生体重児

Outline of Final Research Achievements

The low-birth-weight (LBW) baby have increases due to the in appropriate nourishment, lack of exercise and mental distress in pregnant women. It is thought that LBW baby has been developed to the adult disease; life-style disease, several cancers and mental depression. This finding could be an explanation of the epigenetic effect of the placenta. Firstly, we examined to what factors is associated with the LBW babies. Secondly, we performed genome-wide allelic DNA methylation analyses of purified trophoblast cells from human placentas. RNA sequencing-based allelic expression analyses revealed whether imprinted gene expression is correct or not. We found the LBW risk was associated with smoking and loss of imprinting in their placenta cells (80%).

Research Products

• [Journal Article] Factors associated with aberrant imprint methylation and oligozoospermia (2017)
  • Author(s): Kobayashi N, Miyauchi N, Tatsuta N, Kitamura A, Okae H, Hiura H, Sato A, Utsunomiya T, Yaegashi N, Nakai K, Arima T
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 42336-42336
  • DOI: 10.1038/srep42336
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] DNA methylation errors in imprinting disorders and male infertility. (2015)
  • Author(s): Kitamura A, Hiura H, Miyauchi N, Okae H, Chiba H, Arima T.
  • Journal Title: Congenit Anom (Kyoto). Volume: 55 Pages: 133-144
• [Journal Article] Imprinting methylation errors in ART (2014)
  • Author(s): Hiura H, Okae H, Chiba H, Miyauchi N, Sato F, Sato A, Arima T.
  • Journal Title: Reproductive Medicine and Biology Volume: 13 Pages: 193-202
  • Peer Reviewed / Open Access
• [Presentation] ヒト凍結融解胚移植胎盤におけるmicroRNAの網羅的解析 (2016)
  • Author(s): 樋浦仁、服部裕充、岡江寛明、千葉初音、宮内尚子、北村茜、菊地裕幸、吉田秋仁、有馬隆博
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
• [Presentation] ヒト胚発生におけるエピジェネティクスの変調 (2015)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、千葉初音、宮内尚子
  • Organizer: 第33回受精着床学会
  • Place of Presentation: TFTホール（東京都江東区）
  • Year and Date: 2015-11-26
  • Invited
• [Book] Pharma Medica (2016)
  • Author(s): 有馬隆博、宮内尚子、北村茜、樋浦仁、岡江寛明、千葉初音
  • Total Pages: 144
  • Publisher: メディカルレビュー社
• [Book] 小児科臨床 (2015)
  • Author(s): 樋浦仁、宮内尚子、有馬隆博
  • Total Pages: 8
  • Publisher: 日本小児医事出版社