Epigenetic analyses in patients with imprinting disorders conceived by assisted reproduction technologies
| Project/Area Number |
26870033
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Embryonic/Neonatal medicine
Obstetrics and gynecology
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| Research Institution | Tohoku University |
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Principal Investigator |
Hiura Hitoshi 東北大学, 医学系研究科, 助教 (70451523)
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| Co-Investigator(Renkei-kenkyūsha) |
ARIMA Takahiro 東北大学, 大学院医学系研究科, 教授 (80253532)
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| Research Collaborator |
OGATA Tustomu 浜松医科大学, 医学部, 教授 (40169173)
SOEJIMA Hidenobu 佐賀大学, 医学部, 教授 (30304885)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 生殖補助医療(ART) / インプリント疾患 / DNAメチル化 / 生殖補助医療（ART） / インプリント異常症 |
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| Outline of Final Research Achievements |
The assisted reproduction technologies (ART) are generally considered to be safe, but several studies suggest have reported an increased frequency of imprinting disorder such as Bechwith-Wiedemann syndrome (BWS) and Silver-Russell syndrome (SRS) caused by epigenetic errors in ART children. In this study, to clarify the influence of ART for genome-wide epimutation in SRS and BWS patients derived by ART, we analyzed genome-wide DNA methylation in SRS and BWS patients using reduced representation bisulfite sequencing. In SRS, children derived from ART showed that the frequency and extent of differentially methylated regions in promoters, CpG islands and repeat elements are greater than those of non-ART children. In addition, we could not find mutations had serious effects on DNA methylation-0related genes using exome sequencing. Our data suggest that ART-related risk factor(s) was a process after the fertilization than the gamete formative period.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Factors associated with aberrant imprint methylation and oligozoospermia2017
Author(s)
Kobayashi N, Miyauchi N, Tatsuta N, Kitamura A, Okae H, Hiura H, Sato A, Utsunomiya T, Yaegashi N, Nakai K, Arima T
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Journal Title
Scientific Reports
Volume: 7
Issue: 1
Pages: 42336-42336
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Allele-specific methylome and transcriptome analysis reveals widespread imprinting in the human placenta2016
Author(s)
Hamada, H., Okae, H., Toh, H., Chiba, H., Hiura, H., Shirane, K., Sato, T., Suyama, M., Yaegashi, N., Sasaki, H. & Arima
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Journal Title
The American Journal of Human Genetics
Volume: 99
Issue: 5
Pages: 1045-1058
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Genome-wide analysis of DNA methylation dynamics during early human development.2014
Author(s)
Okae H, Chiba H, Hiura H, Hamada H, Sato A, Utsunomiya T, Kikuchi H, Yoshida H, Tanaka A, Suyama M, Arima T.
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Journal Title
Related Report
Peer Reviewed / Open Access
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[Book] 小児科臨床2015
Author(s)
樋浦仁、宮内尚子、有馬隆博
Total Pages
159
Publisher
日本小児医事出版社
Related Report
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