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Epigenetic analyses in patients with imprinting disorders conceived by assisted reproduction technologies

Research Project

Project/Area Number 26870033
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Embryonic/Neonatal medicine
Obstetrics and gynecology
Research InstitutionTohoku University

Principal Investigator

Hiura Hitoshi  東北大学, 医学系研究科, 助教 (70451523)

Co-Investigator(Renkei-kenkyūsha) ARIMA Takahiro  東北大学, 大学院医学系研究科, 教授 (80253532)
Research Collaborator OGATA Tustomu  浜松医科大学, 医学部, 教授 (40169173)
SOEJIMA Hidenobu  佐賀大学, 医学部, 教授 (30304885)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords生殖補助医療(ART) / インプリント疾患 / DNAメチル化 / 生殖補助医療(ART) / インプリント異常症
Outline of Final Research Achievements

The assisted reproduction technologies (ART) are generally considered to be safe, but several studies suggest have reported an increased frequency of imprinting disorder such as Bechwith-Wiedemann syndrome (BWS) and Silver-Russell syndrome (SRS) caused by epigenetic errors in ART children. In this study, to clarify the influence of ART for genome-wide epimutation in SRS and BWS patients derived by ART, we analyzed genome-wide DNA methylation in SRS and BWS patients using reduced representation bisulfite sequencing. In SRS, children derived from ART showed that the frequency and extent of differentially methylated regions in promoters, CpG islands and repeat elements are greater than those of non-ART children. In addition, we could not find mutations had serious effects on DNA methylation-0related genes using exome sequencing. Our data suggest that ART-related risk factor(s) was a process after the fertilization than the gamete formative period.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (14 results)

All 2017 2016 2015 2014

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) (of which Invited: 1 results) Book (4 results)

  • [Journal Article] Factors associated with aberrant imprint methylation and oligozoospermia2017

    • Author(s)
      Kobayashi N, Miyauchi N, Tatsuta N, Kitamura A, Okae H, Hiura H, Sato A, Utsunomiya T, Yaegashi N, Nakai K, Arima T
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 42336-42336

    • DOI

      10.1038/srep42336

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genome-Scale Assessment of Age-Related DNA Methylation Changes in Mouse Spermatozoa.2016

    • Author(s)
      Kobayashi N, Okae H, Hiura H, Chiba H, Shirakata Y, Hara K, Tanemura K, Arima T.
    • Journal Title

      PLoS One

      Volume: 11 Issue: 11 Pages: e0167127-e0167127

    • DOI

      10.1371/journal.pone.0167127

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Allele-specific methylome and transcriptome analysis reveals widespread imprinting in the human placenta2016

    • Author(s)
      Hamada, H., Okae, H., Toh, H., Chiba, H., Hiura, H., Shirane, K., Sato, T., Suyama, M., Yaegashi, N., Sasaki, H. & Arima
    • Journal Title

      The American Journal of Human Genetics

      Volume: 99 Issue: 5 Pages: 1045-1058

    • DOI

      10.1016/j.ajhg.2016.08.021

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] DNA methylation errors in imprinting disorders and male infertility.2015

    • Author(s)
      Kitamura A, Hiura H, Miyauchi N, Okae H, Chiba H, Arima T.
    • Journal Title

      Congenit Anom (Kyoto).

      Volume: 55 Pages: 133-144

    • Related Report
      2015 Research-status Report
  • [Journal Article] The small leucine-rich repeat secreted protein Asporin induces eyes in Xenopus embryos through the IGF signalling pathway.2015

    • Author(s)
      Luehders K, Sasai N, Davaapil H, Kurosawa-Yoshida M, Hiura H1, Brah T, Ohnuma S.
    • Journal Title

      Development.

      Volume: 142 Issue: 19 Pages: 3351-3361

    • DOI

      10.1242/dev.124438

    • NAID

      120006334951

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Imprinting methylation errors in ART.2014

    • Author(s)
      Hiura H, Okae H, Chiba H, Miyauchi N, Sato F, Sato A, Arima T.
    • Journal Title

      Reproductive Medicine and Biology

      Volume: 13 Pages: 193-202

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome-wide analysis of DNA methylation dynamics during early human development.2014

    • Author(s)
      Okae H, Chiba H, Hiura H, Hamada H, Sato A, Utsunomiya T, Kikuchi H, Yoshida H, Tanaka A, Suyama M, Arima T.
    • Journal Title

      PLOS Genetics

      Volume: 10

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ヒト凍結融解胚移植胎盤におけるmicroRNAの網羅的解析2016

    • Author(s)
      樋浦仁、服部裕充、岡江寛明、千葉初音、宮内尚子、北村茜、菊地裕幸、吉田秋仁、有馬隆博
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 繁殖期におけるマウス精子DNAメチル化様式の加齢性変化2016

    • Author(s)
      小林記緒、岡江寛明、樋浦仁、千葉初音、白形芳樹、原健士郎、種村健太郎、有馬隆博
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒト胚発生におけるエピジェネティクスの変調2015

    • Author(s)
      有馬隆博、樋浦仁、岡江寛明、千葉初音、宮内尚子
    • Organizer
      第33回受精着床学会
    • Place of Presentation
      TFTホール(東京都江東区)
    • Year and Date
      2015-11-26
    • Related Report
      2015 Research-status Report
    • Invited
  • [Book] 生殖補助医療(ART) -胚培養の理論と実際-2017

    • Author(s)
      有馬隆博、樋浦仁、岡江寛明、佐藤晶子
    • Total Pages
      352
    • Publisher
      近代出版
    • Related Report
      2016 Annual Research Report
  • [Book] Pharma Medica2016

    • Author(s)
      有馬隆博、宮内尚子、北村茜、樋浦仁、岡江寛明、千葉初音
    • Total Pages
      144
    • Publisher
      メディカルレビュー社
    • Related Report
      2016 Annual Research Report
  • [Book] 小児科臨床2015

    • Author(s)
      樋浦仁、宮内尚子、有馬隆博
    • Total Pages
      159
    • Publisher
      日本小児医事出版社
    • Related Report
      2015 Research-status Report
  • [Book] エピジェネティクスの産業応用2014

    • Author(s)
      樋浦仁、有馬隆博
    • Total Pages
      9
    • Publisher
      株式会社シーエムシー出版
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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