| Project/Area Number |
26870059
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
Experimental pathology
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| Research Institution | Akita University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | exosome / microRNA / angiogenesis / gallbladder carcinoma |
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| Outline of Final Research Achievements |
Exosomes were isolated from the culture medium of two human gallbladder carcinoma cell lines by means of ultracentrifugation. Exosomes contained miRNAs. Two independent cell lines shared 87 common exosomal miRNAs including miR-125b, miR-320, miR-494, and miR-638, all of which are considered to be anti-angiogenic. NOZ-derived exosomes affected not proliferation but tube formation of HUVEC. The latter was rather inhibited.HUVEC tube formation was suppressed 1 day after miR-494 mimic transfection.
In conclusion, our results have demonstrated that anti-angiogenic miR-494 expressed by human gallbladder cancer NOZ cells is transferred from NOZ cells to HUVECs by the NOZ cell-derived exosomes, and thus suppresses tube formation of HUVECs, suggesting that gallbladder cancer cell-derived exosomes should make pericancerous microenvironment hypoxic to generate a low ROS condition in favor of cell survival and/or to induce cell migration toward normoxic areas.
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