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MicroRNA-containing exosomes derived from human gallbladder cancer cells inhibit HUVEC tube formation

Research Project

Project/Area Number 26870059
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Tumor biology
Experimental pathology
Research InstitutionAkita University

Principal Investigator

Yamamoto Yohei  秋田大学, 医学部, 助教 (70400512)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsexosome / microRNA / angiogenesis / gallbladder carcinoma
Outline of Final Research Achievements

Exosomes were isolated from the culture medium of two human gallbladder carcinoma cell lines by means of ultracentrifugation. Exosomes contained miRNAs. Two independent cell lines shared 87 common exosomal miRNAs including miR-125b, miR-320, miR-494, and miR-638, all of which are considered to be anti-angiogenic. NOZ-derived exosomes affected not proliferation but tube formation of HUVEC. The latter was rather inhibited.HUVEC tube formation was suppressed 1 day after miR-494 mimic transfection.
In conclusion, our results have demonstrated that anti-angiogenic miR-494 expressed by human gallbladder cancer NOZ cells is transferred from NOZ cells to HUVECs by the NOZ cell-derived exosomes, and thus suppresses tube formation of HUVECs, suggesting that gallbladder cancer cell-derived exosomes should make pericancerous microenvironment hypoxic to generate a low ROS condition in favor of cell survival and/or to induce cell migration toward normoxic areas.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (10 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Intra-golgi connexin26 behaves in a pro-oncogenic manner in head and neck cancer cells.2015

    • Author(s)
      Iikawa, N., Yamamoto, Y., Kawasaki, Y., Yoshioka, T., Nishijima, A., Enomoto, K., Ishikawa, K., Omori, Y.
    • Journal Title

      Akita J. Med.

      Volume: 42 Pages: 87-94

    • NAID

      120005678457

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] 血管新生調節因子として機能するヒト胆嚢癌産生exosomeの研究2016

    • Author(s)
      山本洋平,西島亜紀,大森泰文
    • Organizer
      第105回日本病理学会総会
    • Place of Presentation
      仙台市
    • Year and Date
      2016-05-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] MicroRNA-containing exosomes derived from human gallbladder cancer cells inhibit HUVEC tube formation2016

    • Author(s)
      Yamamoto, Y., Nishijima, A., Omori, Y.
    • Organizer
      Annual Meeting - ISEV2016
    • Place of Presentation
      Rotterdam, Netherlands
    • Year and Date
      2016-05-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 腫瘍を抑制するギャップ結合、腫瘍の進展を促すコネキシン2015

    • Author(s)
      大森泰文,川嵜洋平,山本洋平,西島亜紀,飯川延子
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸市
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Can exosomal miRNAs derived from human gallbladder cancer cells regulate angiogenesis?2015

    • Author(s)
      Yamamoto, Y., Nishijima, A., Enomoto, K., Omori, Y.
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] 頭蓋骨の完全欠損を含む多発奇形を伴った羊膜索症候群の一剖検例2015

    • Author(s)
      鈴木麻弥,宮田 元,吉岡年明,山本洋平,西島亜紀,大森泰文
    • Organizer
      第56回日本神経病理学会総会学術研究会
    • Place of Presentation
      福岡市
    • Year and Date
      2015-06-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒト胆嚢癌産生exosome中のmiRNAsが血管新生に与える影響の検討2015

    • Author(s)
      山本洋平、西島亜紀、吉岡年明、榎本克彦、大森泰文
    • Organizer
      日本病理学会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-04-30 – 2015-05-02
    • Related Report
      2014 Research-status Report
  • [Presentation] ヒト胆嚢癌産生exosome中のmiRNAsが血管新生に与える影響の検討2015

    • Author(s)
      山本洋平,西島亜紀,吉岡年明,榎本克彦,大森泰文
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2015-04-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒト前立腺癌幹細胞におけるインテグリンβ4の役割2015

    • Author(s)
      吉岡年明,山本洋平,鈴木麻弥,西島亜紀,南條 博,榎本克彦,大森泰文
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2015-04-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 頭蓋骨の完全欠損を含む多発奇形を伴った羊膜索症候群の一剖検例2015

    • Author(s)
      鈴木麻弥.吉岡年明,山本洋平,西島亜紀,佐藤 朗,大森泰文
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2015-04-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 右血胸から出血性shockに陥った心臓原発血管肉腫の1剖検例2014

    • Author(s)
      山本洋平、吉岡年明、鈴木麻弥、大森泰文
    • Organizer
      日本病理学会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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