Mechanistic analysis of iPSC production using paused iPSC
Project/Area Number |
26870074
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Developmental biology
General medical chemistry
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Research Institution | University of Tsukuba |
Principal Investigator |
Nishimura Ken 筑波大学, 医学医療系, 助教 (80500610)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | Klf4 / iPS細胞 / Tcl1 / 発現量依存的 |
Outline of Final Research Achievements |
We succeeded to produce paused iPSCs with different KLF4 expression levels remaining at distinct intermediate stages of reprogramming. Among the genes highly expressing in Klf4-high paused iPSC, Tcl1 related to induction of high pluripotency. The analyses of Klf4-dose dependent transcriptional regulation in paused iPSC indicated that Klf4 binds to Tcl1 promoter dose dependently and lead to histone modifications, resulting in the induction of Tcl1 expression and pluripotency.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] Genetic variability overrides the impact of parental cell-type and determines iPSCs differentiation potential2016
Author(s)
Kyttala A, Moraghebi R, Valensisi C, Kettunen J, Andrus C, Pasumarthy KK, Nakanishi M, Nishimura K, Ohtaka M, Weltner J, Handel BV, Parkkonen O, Sinisalo J, Jalanko A, Hawkins RD, Woods NB, Otonkoski T, Trokovic R
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Journal Title
Stem Cell Reports
Volume: 6
Issue: 2
Pages: 200-212
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Functional Neurons Generated from T Cell-Derived Induced Pluripotent Stem Cells2016
Author(s)
Matsumoto T, Fujimori K, Andoh-Noda T, Ando T, Kuzumaki N, Toyoshima M, Tada H, Imaizumi K, Ishikawa M, Yamaguchi R, Isoda M, Zhou Z, Sato S, Kobayashi T, Ohtaka M, Nishimura K, Kurosawa H, Yoshikawa T, Takahashi T, Nakanishi M, Ohyama M, Hattori N, Akamatsu W, Okano H.
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Journal Title
Stem Cell Reports.
Volume: 6
Issue: 3
Pages: 422-35
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Interspecific in vitro assay for the chimera-forming ability of human pluripotent stem cells.2015
Author(s)
Masaki H, Kato-Itoh M, Umino A, Sato H, Hamanaka S, Kobayashi T, Yamaguchi T, Nishimura K, Ohtaka M, Nakanishi M, Nakauchi H.
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Journal Title
Development.
Volume: 142(18)
Pages: 3222-30
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A Safeguard System for Induced Pluripotent Stem Cell-Derived Rejuvenated T Cell Therapy.2015
Author(s)
Ando M, Nishimura T, Yamazaki S, Yamaguchi T, Kawana-Tachikawa A, Hayama T, Nakauchi Y, Ando J, Ota Y, Takahashi S, Nishimura K, Ohtaka M, Nakanishi M, Miles JJ, Burrows SR, Brenner MK, Nakauchi H.
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Journal Title
Stem Cell Reports.
Volume: 5(4)
Issue: 4
Pages: 597-608
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Generation of cleidocranial dysplasia-specific human induced pluripotent stem cells in completely serum-, feeder-, and integration-free culture.2015
Author(s)
Yamasaki S, Hamada A, Akagi E, Nakatao H, Ohtaka M, Nishimura K, Nakanishi M, Toratani S, Okamoto T.
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Journal Title
In Vitro Cell Dev Biol Anim.
Volume: 52
Issue: 2
Pages: 252-264
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Prediction of inter-individual differences in hepatic functions and drug sensitivity by using human iPS-derived hepatocytes.2014
Author(s)
Takayama K., Morisaki Y., Kuno S., Nagamoto Y., Harada K., Furukawa N., Ohtaka M.,Nishimura K., Imagawa K., Sakurai F., Tachibana M., Sumazaki R., Noguchi E., Nakanishi M., Hirata K., Kawabata K., Mizuguchi H.
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 111
Issue: 47
Pages: 16772-7
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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