The effects of intestinal microbiota on host-pathogen interaction during malaria infection

• Project/Area Number: 26870094
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Epidemiology and preventive medicine; Parasitology (including sanitary zoology)
• Research Institution: Gunma University
• Principal Investigator: Taniguchi Tomoyo 群馬大学, 大学院医学系研究科, 助教 (20620800)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: マラリア / 熱帯熱マラリア / 消化器症状 / 腸内細菌叢 / 腸内細菌 / Plasmodium falciparum / dysbiosis / ネズミマラリア / 脳マラリア / Plasmodium berghei ANKA / 腸管病態

Outline of Final Research Achievements

Gastrointestinal symptoms are frequently observed in malaria patients in addition to the malarial triad of fever, anemia, and splenomegaly. However, the association between intestinal microbiota and malaria pathology is poorly understood. In this study, we revealed that mice infected with Plasmodium berghei ANKA suffer from intestinal pathology in the small intestines associated with remarkable changes in microbiota. Microbiotic analyses of stool samples also revealed that P. falciparum patients have a different microbiota composition compared with healthy controls. Furthermore, some genera or clusters of microbiota in malaria patients and infected mice correlated with malaria pathology including gastrointestinal symptoms. These results indicate that intestinal microbiota may affect malaria pathogenesis.

Research Products

• [Int'l Joint Research] グル大学/St. Mary’s Hospital Lacor, Gulu(ウガンダ)
• [Journal Article] Asymptomatic malaria, growth status, and anaemia among children in Lao People's Democratic Republic: a cross-sectional study (2016)
  • Author(s): Takeshi Akiyama, Tiengkham Pongvongsa, Souraxay Phrommala, Tomoyo Taniguchi, Yuba Inamine, Rie Takeuchi, Tadashi Watanabe, Futoshi Nishimoto, Kazuhiko Moji, Shigeyuki Kano, Hisami Watanabe, and Jun Kobayashi
  • Journal Title: Malaria Journal Volume: 15 Issue: 1 Pages: 499-499
  • DOI: 10.1186/s12936-016-1548-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Plasmodium berghei ANKA causes intestinal malaria associated with dysbiosis (2015)
  • Author(s): Taniguchi T, Miyauchi E, Nakamura S, Hirai M, Suzue K, Imai T, Nomura T, Handa T, Okada H, Shimokawa C, Onishi R, Olia A, Hirata J, Tomita H, Ohno H, Horii T, Hisaeda H
  • Journal Title: Scientific Reports Volume: 5:15699 Issue: 1 Pages: 1-12
  • DOI: 10.1038/srep15699
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A transient resistance to blood-stage malaria in interferon-γ-deficient mice through impaired production of the host cells preferred by malaria parasites. (2015)
  • Author(s): Okada H, Suzue K, Imai T, Taniguchi T, Shimokawa C, Onishi R, Hirata J, Hisaeda H.
  • Journal Title: Front Microbiol. Volume: 6 Pages: 600-600
  • DOI: 10.3389/fmicb.2015.00600
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cytotoxic activities of CD8+ T cells collaborate with macrophages to protect against blood-stage murine malaria (2015)
  • Author(s): Imai T, Ishida H, Suzue K, Taniguchi T, Okada H, Shimokawa C, and Hisaeda H
  • Journal Title: eLIFE Volume: 4 Pages: 4-4
  • DOI: 10.7554/elife.04232
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evaluating experimental cerebral malaria using oxidative stress indicator OKD48 mice. (2014)
  • Author(s): Imai T, Iwawaki T, Akai R, Suzue K, Hirai M, Taniguchi T, Okada H, Hisaeda H.
  • Journal Title: Int J Parasitol. Volume: 44 Issue: 10 Pages: 681-685
  • DOI: 10.1016/j.ijpara.2014.06.002
  • Peer Reviewed / Open Access
• [Presentation] ウガンダ国の熱帯熱マラリア患者において腸内細菌叢の変化が起こる (2017)
  • Author(s): 谷口委代、宮内栄治、Olia Alex、長安英治、Osbert Katuro、鈴江一友、今井孝、下川周子、大西里咲、Odongo-Aginya E.I.、Palacpac Niriann、丸山治彦、木村英作、美田敏宏、大野博司、堀井俊宏、久枝一
  • Organizer: 第86回日本寄生虫学会大会
  • Place of Presentation: 北海道大学（北海道・札幌）
  • Year and Date: 2017-05-28
• [Presentation] Altered gut microbiota composition in Plasmodium falciparum patients in Uganda (2017)
  • Author(s): Taniguchi T, Miyauchi E, Nakamura S, Hirai M, Suzue K, Imai T, Nomura T, Handa T, Okada H, Shimokawa C, Onishi R, Olia A, Hirata J, Tomita H, Ohno H, Horii T and Hisaeda H
  • Organizer: Japanese-Brazil Malaria Workshop 2017
  • Place of Presentation: サンパウロ（ブラジル）
  • Year and Date: 2017-03-15
  • Int'l Joint Research
• [Presentation] Plasmodium berghei ANKA infection induces intestinal dysbiosis (2016)
  • Author(s): Taniguchi T, Miyauchi E, Nakamura S, Hirai M, Suzue K, Imai T, Nomura T, Handa T, Okada H, Shimokawa C, Onishi R, Olia A, Hirata J, Tomita H, Ohno H, Horii T and Hisaeda H
  • Organizer: International Congress of Immunology
  • Place of Presentation: メルボルン（オーストラリア）
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Pathogen non-specific bystander T cells were attenuated upon infection with malaria parasites (2016)
  • Author(s): Suzue K, Amano H, Taniguchi T, Shimokawa C, Olia A, Onishi R, and Hisaeda H
  • Organizer: International Congress of Immunology
  • Place of Presentation: メルボルン（オーストラリア）
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Plasmodium berghei ANKA infection induces intestinal dysbiosis (2016)
  • Author(s): Tomoyo Taniguchi, Eiji Miyauchi, Shota Nakamura, Makoto Hirai, Kazutomo Suzue, Takashi Imai, Takahiro Nomura, Tadashi Handa, Hiroko Okada, Chikako Shimokawa, Risa Onishi , Alex Olia, Jun Hirata, Haruyoshi Tomita, Hiroshi Ohno, Toshihiro Horii and Hajime Hisaeda
  • Organizer: ICI2016
  • Place of Presentation: メルボルン（オーストラリア）
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] ネズミマラリア原虫感染により腸内細菌バランス失調が起こる (2016)
  • Author(s): 谷口委代、宮内栄治、中村昇太、平井誠、鈴江一友、今井孝、岡田紘子、下川周子、大西里咲、Alex Olia、大野博司、堀井俊宏、久枝一
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎）
  • Year and Date: 2016-03-19
• [Presentation] Plasmodium falciparum malaria alters the composition of intestinal microbiota in Ugandan patients (2016)
  • Author(s): Taniguchi T, Miyauchi E, Olia A, Nagayasu E, Sakurai M, Ikeda M, Imai T, Shimokawa C, Hirata J, Odongo-Aginya EI, Palacpac N, Maruyama H, Kimura E, Mita T, Ohno H, Horii T and Hisaeda H
  • Organizer: TICAD VI Pre-event; The 3rd International Symposium for the Promotion of Science and Technology Innovation Cooperation between Africa and Japan
  • Place of Presentation: JICA Ichigaya Building（東京都・市谷本村町）
  • Int'l Joint Research
• [Presentation] Dysbiosis Associated with Intestinal Pathology in Mice Infected with Malaria Parasites. (2015)
  • Author(s): Tomoyo Taniguchi, Eiji Miyauchi, Hiroshi Ohno and Hajime Hisaeda
  • Organizer: ICMI 2015
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2015-07-14 – 2015-07-18
• [Presentation] ネズミマラリアにおける腸管病変と腸内細菌叢の変化 (2015)
  • Author(s): 谷口委代、宮内栄治、中村昇太、平井誠、鈴江一友、今井孝、岡田紘子、大野博司、堀井俊宏、久枝一
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-21 – 2015-03-22
• [Remarks] 群馬大学大学院医学系研究科国際寄生虫病学
  • URL: http://www.med.gunma-u.ac.jp/med-organization/envmed/envmed-defense/157.html/
• [Remarks] マラリア感染により腸内細菌叢が悪化する ‐マラリアの新たな予防・治療法開発への道を拓く‐
  • URL: http://www.gunma-u.ac.jp/information/10217