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Molecular function of Maelstrom in germline piRNA biogenesis pathway in Bombyx mori

Research Project

Project/Area Number 26870112
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Molecular biology
Genome biology
Research InstitutionThe University of Tokyo

Principal Investigator

Sato Kaoru  東京大学, 理学(系)研究科(研究院), 助教 (20548507)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordspiRNA / Maelstrom / Transposon / RNA scilencing / Germline / Silkworm / Piwi / RNAi / トランスポゾン / RNAサイレンシング / 生殖 / ゲノム / RNA
Outline of Final Research Achievements

piRNA (PIWI-interacting RNA) is a subset of small RNAs specifically produced in animal gonad, and associates with PIWI proteins to suppress the expression of transposons. BmN4 cell is derived from silkworm ovarian germ cell, and is a useful culture system to studying germline piRNA biogenesis. In this project, I examined the molecular function of an piRNA factor Maelstrom (Mael) in the germline piRNA biogenesis in silkworm. Especially, I aimed to identify the Mael-interacting RNA and proteins. First, to isolate the Mael-interacting RNAs, I performed CLIP analysis using anti-Mael antibody, and detected RNAs which was crosslinked with Mael. Now, I am trying to prepare the RNA library for deep sequence. Next, I identified a silkworm PIWI protein Siwi and two piRNA factors, BmSpn-E and BmQin in the Mael-complexes. Now, I am analyzing Mael-complex formation and cellular localization when each piRNA factor is depleted in BmN4 cells.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (12 results)

All 2015 2014 Other

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Tudor-domain containing proteins act to make the piRNA pathways more robust in Drosophila2015

    • Author(s)
      Sato K, Iwasaki YW, Siomi H, Siomi MC
    • Journal Title

      Fly

      Volume: 9 Issue: 2 Pages: 86-90

    • DOI

      10.1080/19336934.2015.1128599

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Krimper Enforces an Antisense Bias on piRNA Pools by Binding AGO3 in the Drosophila Germline2015

    • Author(s)
      Sato K, Iwasaki YW (equal contribution), Shibuya A, Carninci P, Tsuchizawa Y, Ishizu H, Siomi MC, Siomi H
    • Journal Title

      Molecular Cell

      Volume: 59 Issue: 4 Pages: 553-563

    • DOI

      10.1016/j.molcel.2015.06.024

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Crystal Structure and Activity of the Endoribonuclease Domain of the piRNA Pathway Factor Maelstrom2015

    • Author(s)
      Naoki Matsumoto, Kaoru Sato, Hiroshi Nishimasu, Yurika Namba, Kana Miyakubi, Naoshi Dohmae, Ryuichiro Ishitani, Haruhiko Siomi, Mikiko C. Siomi, Osamu Nureki
    • Journal Title

      Cell Reports

      Volume: 11 Issue: 3 Pages: 1-10

    • DOI

      10.1016/j.celrep.2015.03.030

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functional and structural insights into the piRNA factor Maelstrom.2015

    • Author(s)
      Sato K and Siomi MC.
    • Journal Title

      FEBS Letters

      Volume: 589 Issue: 14 Pages: 1688-1693

    • DOI

      10.1016/j.febslet.2015.03.023

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] PIWI-interacting RNAを介したトランスポゾン転写抑制機構2015

    • Author(s)
      大西遼、佐藤薫、塩見美喜子
    • Journal Title

      ナノ学会会報

      Volume: 13 Pages: 61-71

    • Related Report
      2014 Research-status Report
  • [Presentation] Distinctive functions of Maelstrom in the piRNA pathway in the germline and ovarian somatic cells2015

    • Author(s)
      Kaoru Sato, Yurika Namba, Oonishi Ryo, Mikiko C. Siomi
    • Organizer
      RNA Japan 2015
    • Place of Presentation
      Hotel Lifort Sapporo (Sapporo, JAPAN)
    • Year and Date
      2015-07-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] Essential roles of a Tudor domain-containing protein, Krimper, in piRNA biogenesis in Drosophila ovaries2015

    • Author(s)
      Sato K, Iwasaki YW, Shibuya A, Carninci P, Ishizu H, Siomi MC, Siomi H.
    • Organizer
      RNA 2015
    • Place of Presentation
      Madison, Wisconsin, USA.
    • Year and Date
      2015-05-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ショウジョウバエ生殖細胞におけるpiRNA生合成機構2014

    • Author(s)
      佐藤薫、塩見美喜子
    • Organizer
      分子研研究会「細胞核内反応の分子科学」
    • Place of Presentation
      岡崎コンファレンスセンター、岡崎、日本
    • Year and Date
      2014-09-27
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Distinctive functions of Maelstrom in the piRNA pathway in Drosophila germline and ovarian somatic cells2014

    • Author(s)
      Kaoru Sato, Namba Yurika, Ryo Ohnishi, Haruhiko Siomi and Mikiko C Siomi
    • Organizer
      IIAS Research Conference 2014 ”Chromatin Decoding”
    • Place of Presentation
      IIAS, Nara, JAPAN
    • Year and Date
      2014-05-12 – 2014-05-15
    • Related Report
      2014 Research-status Report
  • [Book] ナノ学会会報、PIWI-interacting RNAを介したトランスポゾン転写抑制機構2015

    • Author(s)
      大西遼、佐藤薫、塩見美喜子
    • Total Pages
      5
    • Publisher
      ナノ学会
    • Related Report
      2015 Annual Research Report
  • [Remarks] KrimpはAGO3とセンスpiRNAとの相互作用を制御する(以下略)

    • URL

      http://first.lifesciencedb.jp/archives/10567

    • Related Report
      2015 Annual Research Report
  • [Remarks] 東京大学大学院理学系研究科生物科学専攻塩見研究室研究業績

    • URL

      http://www-siomilab.biochem.s.u-tokyo.ac.jp/index.html

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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