| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
piRNA (PIWI-interacting RNA) is a subset of small RNAs specifically produced in animal gonad, and associates with PIWI proteins to suppress the expression of transposons. BmN4 cell is derived from silkworm ovarian germ cell, and is a useful culture system to studying germline piRNA biogenesis. In this project, I examined the molecular function of an piRNA factor Maelstrom (Mael) in the germline piRNA biogenesis in silkworm. Especially, I aimed to identify the Mael-interacting RNA and proteins. First, to isolate the Mael-interacting RNAs, I performed CLIP analysis using anti-Mael antibody, and detected RNAs which was crosslinked with Mael. Now, I am trying to prepare the RNA library for deep sequence. Next, I identified a silkworm PIWI protein Siwi and two piRNA factors, BmSpn-E and BmQin in the Mael-complexes. Now, I am analyzing Mael-complex formation and cellular localization when each piRNA factor is depleted in BmN4 cells.
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