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Metabolic regulation via protein acylation in Thermus thermophilus

Research Project

Project/Area Number 26870113
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied biochemistry
Applied microbiology
Research InstitutionThe University of Tokyo

Principal Investigator

Yoshida Ayako  東京大学, 生物生産工学研究センター, 特任助教 (90633686)

Co-Investigator(Renkei-kenkyūsha) KOSONO Saori  東京大学, 生物生産工学研究センター, 特任准教授 (90321760)
NISHIYAMA Makoto  東京大学, 生物生産工学研究センター, 教授 (00208240)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsタンパク質アシル化修飾 / タンパク質アセチル化酵素 / アミノ酸代謝 / CoA体代謝 / 代謝調節 / タンパク質間相互作用 / タンパク質相互作用 / タンパク質翻訳後修飾 / 短鎖アシル化 / Thermus thermophilus
Outline of Final Research Achievements

The proteomic analysis revealed that a lot of proteins were acetylated and succinylated in Thermus thermophilus HB27. 2-isopropylmalate synthase (IPMS), which catalyzes the first step of leucine biosynthesis, is both acetylated and succinylated. The acylation-mimic mutant showed the lower activity and changed the substrate specificity. We suppose that the acylation on IPMS may regulate the flux of branched-chain amino acids.
The analysis of protein lysine acetyltransferase (KAT) from T. thermophilus identified the CoA transferase as an acetylation substrate of KAT. Since this CoA transferase uses Acetyl-CoA, which is a substrate of KAT, suggesting that the acetylation of CoA transferase regulates CoA metabolism depending on the concentration of Acetyl-CoA. Moreover, we found the protein interacting with this CoA transferase, suggesting that the activity of CoA transferase is regulated in a complex manner including protein acylation and regulatory protein binding.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (12 results)

All 2016 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (7 results) Remarks (2 results)

  • [Journal Article] Altered acetylation and succinylation profiles in Corynebacterium glutamicum in response to conditions inducing glutamate overproduction.2016

    • Author(s)
      Mizuno Y., Nagano-Shoji M., Kubo S., Kawamura Y., Yoshida A., Kawasaki H., Nishiyama M., Yoshida M. and Kosono S.
    • Journal Title

      MicrobiologyOpen

      Volume: 5 Issue: 1 Pages: 152-173

    • DOI

      10.1002/mbo3.320

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Changes in the acetylome and succinylome of Bacillus subtilis in response to carbon source.2015

    • Author(s)
      Kosono S., Tamura M., Suzuki S., Kawamura Y., Yoshida A., Nishiyama M., Yoshida M.
    • Journal Title

      PLoS One

      Volume: 10 Issue: 6 Pages: e0131169-e0131169

    • DOI

      10.1371/journal.pone.0131169

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Structural insight into amino group-carrier protein-mediated lysine biosynthesis: crystal structure of the LysZ•LysW complex from Thermus thermophilus2015

    • Author(s)
      Ayako Yoshida, Takeo Tomita, Tsutomu Fujimura, Chiharu Nishiyama, Tomohisa Kuzuyama, and Makoto Nishiyama
    • Journal Title

      Journal of Biological Chemistry

      Volume: 290 Issue: 1 Pages: 435-447

    • DOI

      10.1074/jbc.m114.595983

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Thermus thermophilusにおいてアセチル化を受けるCoAトランスフェラーゼの機能と制御機構の解析2016

    • Author(s)
      山本寛之、吉田彩子、富田武郎、古園さおり、葛山智久、西山真
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 枯草菌ECFシグマ因子SigXのグルコース依存的な活性化に関わるRNAポリメラーゼ のアセチル化2016

    • Author(s)
      米沢祐大、小倉光雄、鈴木祥太、吉田彩子、浅井計、吉田稔、西山真、古園さおり
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Corynebacterium glutamicum由来ホスホエノールピルビン酸カルボキシラーゼ(PEPC)のアセチル化制御はグルタミン酸生産に重要である2016

    • Author(s)
      永野愛、山田鮎果、水野裕太、菊池正樹、白水美香子、梅原崇史、吉田彩子、吉田稔、西山真、古園さおり
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 高度好熱菌Thermus thermophilusにおけるタンパク質アセチル化酵素及び標的タンパク質の同定と機能解析2015

    • Author(s)
      山本寛之、吉田彩子、富田武郎、古園さおり、葛山智久、西山真
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学(岡山県岡山市)
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 高度好熱菌Thermus thermophilusにおける短鎖アシル化修飾による分岐鎖アミノ酸生合成酵素の調節2014

    • Author(s)
      吉田彩子、西山真、吉田稔、古園さおり
    • Organizer
      第15回極限環境生物学会年会
    • Place of Presentation
      今帰仁村コミュニティーセンター(沖縄県今帰仁村)
    • Year and Date
      2014-11-01 – 2014-11-03
    • Related Report
      2014 Research-status Report
  • [Presentation] Analysis of protein acylation on metabolic enzymes and acyltransferase in Thermus thermophilus HB272014

    • Author(s)
      吉田彩子、山本寛之、富田武郎、西山真、吉田稔、古園さおり
    • Organizer
      第四回モデル生物丸ごと一匹学会
    • Place of Presentation
      大阪大学(大阪府豊中市)
    • Year and Date
      2014-09-26 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 高度好熱菌におけるアシル化修飾による代謝酵素調節機構の解析2014

    • Author(s)
      吉田彩子
    • Organizer
      第三回育志賞研究発表会
    • Place of Presentation
      東京工業大学(東京都目黒区)
    • Year and Date
      2014-08-20
    • Related Report
      2014 Research-status Report
  • [Remarks] 微生物機能代謝工学研究室ホームページ

    • URL

      http://part.itc.u-tokyo.ac.jp/biotec-res-ctr/MBT/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 微生物機能代謝工学研究室ホームページ

    • URL

      http://park.itc.u-tokyo.ac.jp/biotec-res-ctr/MBT/

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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