Development of measles virus vector-based vaccine against highly pathogenic avian influenza virus

• Project/Area Number: 26870147
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science; Virology
• Research Institution: The University of Tokyo
• Principal Investigator: Fujiyuki Tomoko 東京大学, 医科学研究所, 助教 (50610630)
• Research Collaborator: KAI Chieko 東京大学, 医科学研究所, 教授 (10167330) ; YONEDA Misako 東京大学, 医科学研究所, 准教授 (40361620) ; KOHARA Michinori 東京都医学総合研究所, ゲノム医科学研究分野, シニア研究員 (10250218) ; KIDA Hiroshi 北海道大学, 人獣共通感染症リサーチセンター, 特任教授 (10109506) ; HATTORI Shosaku 東京大学, 医科学研究所, 特任研究員 (00164864)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 高病原性鳥インフルエンザウイルス / 麻疹ウイルス / カニクイザル / 感染モデル / 発症防御 / ワクチン / ベクター / 組換えウイルス / ウイルスベクター / H5N1

Outline of Final Research Achievements

We generated recombinant measles virus (rMV) expressing the hemagglutinin (HA) protein of HPAIV (H5), and examined protective efficacy of the candidate vaccine in the monkey infection model by the challenge with a HPAIV (H5N1). Cynomolgus monkeys inoculated with the candidate vaccine produced anti-H5 HA antibodies and recovered earlier from influenza symptoms than unvaccinated monkeys after the challenge with the HPAIV strain. In addition, vaccination tended to suppress viral shedding. Moreover, even in monkeys possessing pre-existing anti-MV antibodies, the vaccination with the candidate induced the production of antibodies against H5 HA. These results suggest that the rMV expressing H5 HA is a good candidate vaccine against HPAIV infection to reduce disease severity.

Research Products

• [Presentation] 麻疹ウイルスベクターを利用した高病原性鳥インフルエンザウイルスワクチンの霊長類感染モデルにおける効果 (2016)
  • Author(s): 藤幸知子、堀江亮、米田美佐子、倉石武、安井文彦、権賢貞、池田房子、宗片圭祐、石井美穂、木曽有里、佐藤宏樹、服部正策、喜田宏、小原道法、甲斐知恵子
  • Organizer: 第６３回日本実験動物学会
  • Place of Presentation: 川崎
  • Year and Date: 2016-05-18
• [Presentation] Efficacy of recombinant measles virus expressing highly pathogenic avian influenza virus antigen in non-human primate model (2015)
  • Author(s): Tomoko Fujiyuki, Ryo Horie, Misako Yoneda, Takeshi Kuraishim Fumihiko Yasui, Hyun-jeoung Kwon, Fusako Ikeda, Keisuke Munekata, Miho Ishii, Yuri Kiso, Mio Omi, Hiroki Sato, Hiroshi Kida, Shosaku Hattori, Michinori Kohara, Chieko Kai.
  • Organizer: 第６３回日本ウイルス学会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2015-11-22
• [Presentation] H5N1高病原性鳥インフルエンザ組換えワクチニアワクチン単回接種による免疫長期持続作用機序の解析 (2014)
  • Author(s): 安井文彦、宗片圭祐、倉石武、服部正策、藤幸知子、米田美佐子、迫田義博、喜田宏、甲斐知恵子、小原道法
  • Organizer: 第６２回日本ウイルス学会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-10
• [Presentation] Attenuated recombinant measles virus expressing highly pathogenic avian influenza virus (HPAIV) antigen is protective against HPAIV challenge in cynomolgus monkeys (2014)
  • Author(s): Tomoko Fujiyuki, Ryo Horie, Misako Yoneda, Takeshi Kuraishi, Fumihiko Yasui, Keisuke Munekata, Shosaku Hattori, Hiroshi Kida, Michinori Kohara, Chieko Kai.
  • Organizer: XVIth International Congress of Virology
  • Place of Presentation: カナダ、モントリオール
  • Year and Date: 2014-07-27 – 2014-08-01
• [Presentation] 霊長類感染モデルを用いた高病原性鳥インフルエンザウイルス抗原発現組換え麻疹ウイルスの防御効果の解析 (2014)
  • Author(s): 藤幸知子、堀江亮、米田美佐子、倉石武、安井文彦、宗片圭祐、池田房子、木曽有里、権賢貞、石井美穂、佐藤宏樹、服部正策、喜田宏、小原道法、甲斐知恵子
  • Organizer: 第６１回日本実験動物学会
  • Place of Presentation: 札幌
  • Year and Date: 2014-05-15 – 2014-05-17
• [Presentation] Macaque models of highly pathogenic avian influenza virus infection using a wild water bird-derived strain. (2014)
  • Author(s): Tomoko Fujiyuki, Misako Yoneda, Fumihiko Yasui, Takeshi Kuraishi, Shosaku Hattori, Hyun-jeong Kwon, Keisuike Munekata, Yuri Kiso, Hiroshi Kida, Michinori Kohara, Chieko Kai.
  • Organizer: 第１４回東京大学生命科学シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2014-04-26