Investigation about the theraputic effects of omega-3 fatty acids in endometriosis

• Project/Area Number: 26870148
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry; Obstetrics and gynecology
• Research Institution: Research Institute for Clinical Oncology, Saitama Cancer Center
• Principal Investigator: TOMIO KENSUKE 埼玉県立がんセンター(臨床腫瘍研究所), その他部局等, その他 (40707254)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: オメガ３脂肪酸 / 子宮内膜症 / 抗炎症作用 / マクロファージ / インフラマソーム / 脂肪酸代謝物

Outline of Final Research Achievements

A new therapeutic method is highly anticipated for endometriosis, a chronic inflammatory disease. In the present study, the inhibitory effect of omega-3 fatty acids (ω3FAs) on endometriosis was verified in vitro. LPS-stimulation of cultured peritoneal macrophages (Mφ) from endometriosis patients with ω3FAs present did not inhibit TNF-α production. It was thus focused on how ω3FAs reduce IL-1β production by inhibiting Mφ inflammasome activity. After treatment with ω3FAs, IL-1β production from cultured peritoneal Mφ in response to stimulation with nigericin (a reagent for inflammasome activation) was inhibited. These results suggested that ω3FAs suppress inflammation in endometriosis by inhibiting the Mφ inflammasome activity, a finding which could lead to a novel therapeutic strategy.

Research Products

• [Journal Article] Systemic Delivery of Sirna by Actively Targeted Polyion Complex Micelles for Silencing the E6 and E7 Human Papillomavirus Oncogenes (2016)
  • Author(s): Nishida H, Matsumoto Y, Kawana K, Christie RJ, Naito M, Kim BS, Toh K, Min HS, Yi Y, Matsumoto Y, Kim HJ, Miyata K, Taguchi A, Tomio K, Yamashita A, Inoue T, Nakamura H, Fujimoto A, Sato M, Yoshida M, Adachi K, Arimoto T, Wada-Hiraike O, Oda K, Nagamatsu T, Nishiyama N, Kataoka K, Osuga Y, Fujii T
  • Journal Title: J Control Release Volume: in press Pages: 29-37
  • DOI: 10.1016/j.jconrel.2016.03.016
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Decreased expression of the plasminogen activator inhibitor type 1 is involved in degradation of extracellular matrix surrounding cervical cancer stem cells. (2016)
  • Author(s): Sato M, Kawana K, Adachi K, Fujimoto A, Yoshida M, Nakamura H, Nishida H, Inoue T, Taguchi A, Takahashi J, Kojima S, Yamashita A, Tomio K, Nagamatsu T, Wada-Hiraike O, Oda K, Osuga Y, Fujii T.
  • Journal Title: Int J Oncol Volume: 48 Issue: 2 Pages: 829-835
  • DOI: 10.3892/ijo.2015.3283
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Radical hysterectomy with or without para-aortic lymphadenectomy for patients with stage IB2, IIA2, and IIB cervical cancer: outcomes for a series of 308 patients. (2016)
  • Author(s): Tsuruga T, Fujimoto A, Kawana K, Mori M, Hasumi Y, Kino N, Tomio K, Miura S, Tanikawa M, Sone K, Miyamoto Y, Ikeda Y, Kojima S, Adachi K, Nagasaka K, Matsumoto Y, Arimoto T, Oda K, Nakagawa S, Horie K, Yasugi T, Yokota H, Osuga Y, Fujii T
  • Journal Title: Int J Clin Oncol Volume: in press Issue: 2 Pages: 359-366
  • DOI: 10.1007/s10147-015-0907-3
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Positive peritoneal cytology at interval debulking surgery is independent poor prognostic factor in patients with stage T3c advanced ovarian carcinoma (2014)
  • Author(s): Nagasaka K, Kawana K, Tomio K, Eguchi S, Miura S, Tsuruga T, Mori-Uchino M, Tanikawa M, Tsukazaki T, Sone K, Adachi K, Matsumoto Y, Arimoto T, Oda K, Osuga Y, Fujii T
  • Journal Title: J Obstet Gynecol Res Volume: Nov. 25 Issue: 5 Pages: 554-560
  • DOI: 10.1111/jog.12616
  • Peer Reviewed
• [Journal Article] Oral vaccination against HPV E7 for treatment of cervical intraepithelial neoplasia grade 3 (CIN3) elicits E7-specific mucosal immunity in the cervix of CIN3 patients (2014)
  • Author(s): Kawana K, Adachi K, Kojima S, Taguchi A, Tomio K, Yamashita A, Nishida H, Nagasaka K, Arimoto T, Yokoyama T, Wada-Hiraike O, Oda K, Sewaki T, Osuga Y, Fujii T
  • Journal Title: Vaccine Volume: 32 Issue: 47 Pages: 6233-6239
  • DOI: 10.1016/j.vaccine.2014.09.020
  • Peer Reviewed
• [Presentation] 癌遺伝子(ras、myc)は癌微小環境に働きかけ卵巣癌の腹腔内の癌進展様式を変化させる (2015)
  • Author(s): 吉田光代 田口歩 川名敬 冨尾賢介 中村寛江 藤本麻葉 山下亜紀 長阪一憲 足立克之 織田克利 清野透 大須賀穣 藤井知行
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] Resveratrol has a therapeutic potential for treating endometriosis (2015)
  • Author(s): Ayumi Taguchi, Kaori Koga, Kei Kawana, Osamu Hiraike, Mariko Miyashita, Fusako Sue, Tomoko Makabe, Aki Yamashita, Kensuke Tomio, Tomoyuki Fujii and Yutaka Osuga.
  • Organizer: 国際生殖医学会・日本生殖医学会学術集会2015
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2015-04-26 – 2015-04-30
• [Presentation] Resveratrol has a therapeutic potential for treating endometriosis (2015)
  • Author(s): Ayumi Taguchi, Kaori Koga, Kei Kawana, Osamu Hiraike, Mariko Miyashita, Fusako Sue, Tomoko Makabe, Aki Yamashita, Kensuke Tomio, Tomoyuki Fujii and Yutaka Osuga
  • Organizer: International Federation of Fertility Societies International Meeting 2015
  • Place of Presentation: 横浜
  • Year and Date: 2015-04-26
  • Int'l Joint Research
• [Presentation] サバイビンをターゲットとした子宮内膜症治療方法～レスベラトロール～ (2015)
  • Author(s): 田口歩 甲賀かをり 川名敬 平池修 宮下真理子 須江英子 真壁友子 山下亜紀 冨尾賢介 大須賀穣 藤井知行
  • Organizer: 第36回日本エンドメトリオーシス学会学術集会
  • Place of Presentation: ハイアットリージェンシー東京（新宿区）
  • Year and Date: 2015-01-24 – 2015-01-25