| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We previously showed that functional islets with a 3-dimensional structure consisting of multiple endocrine cells were formed in vitro from mouse fetal pancreatic bud cells. Based on this finding, we have developed a culture system to generate functional islet from human iPS cells. In this study, we isolate highly enriched population of pancreatic endocrine progenitor cells from endocrine progenitor cell culture stage cells by cell sorting. These cells can differentiate into the functional islet-like cell cluster. This result indicate that the iPS-islets can be generated from endocrine cells efficiently. Furthermore, we found that Ngn3 positive cells formed islet-like cell aggregate autonomously in this differentiation system. We are currently trying to scale up the culture system for production of a large quantity of islets.
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