Effect of oxygen nanobubbles water on avulsed and replanted teeth for conservative therapy

• Project/Area Number: 26870173
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry; Orthodontics/Pediatric dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: OISHI Atsushi 東京医科歯科大学, 歯学部附属病院, 医員 (50645166)
• Research Collaborator: TAKAHASHI Masayoshi 産業総合研究所, 主任研究員 ; ARAKAWA Shinichi 東京医科歯科大学, 生涯口腔保健衛生学分野, 教授 ; SHIMOKAWA Hitoyata 東京医科歯科大学, 小児歯科学分野, 非常勤講師
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ナノバブル / マイクロバブル / ウルトラファインバブル / 歯の外傷 / 歯小嚢 / 細胞培養 / 酸素 / オゾン / ヒト口腔由来細胞 / 細胞増殖 / マイクロバブル・ナノバブル / 生理活性作用

Outline of Final Research Achievements

This study investigated the efficacy of oxygen nanobubble water to encourage proper healing of traumatized teeth. In the beginning of this study, previous studies about nanobubble water reported its bioactive effect by the increase of survival rate etc, and the mechanism to living organisms was not fully elucidated. Additionally, renovation of our animal laboratory building was postponed. Therefore, our initial experiment plan required significant change, and we inspected the effect of nanobubble water on human oral tissue as a previous step of therapeutic application of nanobubble water to traumatized teeth. Oxygen and air nanobubble water inhibited the proliferation of human dental follicle stem cells in vitro. However, comprehensive elucidation of the effects of air and oxygen nanobubble water on oral tissue and living organisums will require further investigation both in vitro and in vivo.

Research Products

• [Presentation] Effects of Nanobubble water on human cells derived from impacted supernumerary teeth. (2017)
  • Author(s): Atsushi Oishi, Hitoyata Shimokawa, Wit Yee Wint, Shinichi Arakawa, Michiyo Miyashin
  • Organizer: 10th Biennial Conference of the Pediatric Dentistry Association of Asia in conjunction with 54th Annual Conference of the Japanese Society of Pediatric Dentistry
  • Int'l Joint Research
• [Presentation] Effects of Nanobubble water on human cells derived from impacted supernumerary teeth. (2016)
  • Author(s): Oishi Atsushi, Shimokawa Hitoyata, Wit Yee Wint, Arakawa Shinichi, Miyashin Michiyo
  • Organizer: 10th Biennial Conference of the Pediatric Dentistry Association of Asia in conjunction with 54th Annual Conference of the Japanese Society of Pediatric Dentistry
  • Place of Presentation: 東京都文京区 東京ドームホテル
  • Year and Date: 2016-05-27
  • Int'l Joint Research
• [Presentation] Effects of Nanobubble water on human cells derived from impacted supernumerary teeth (2016)
  • Author(s): Atsushi Oishi, Hitoyata Shimokawa, Wit Yee Wint, Shinichi Arakawa, Michiyo Miyashin
  • Organizer: 10th Biennial Conference of the Pediatric Dentistry Association of Asia in conjunction with 54th Annual Conference of the Japanese Society of Pediatric Dentistry
  • Place of Presentation: 東京ドームホテル（東京都文京区）
  • Year and Date: 2016-05-27
  • Int'l Joint Research