| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We have achieved direct chemical derivatization of natural plant extracts, where electrophilic ketones in the extracts could be selectively converted into the corresponding hydantoins (imidazolidine-2,4-dione) under the Bucherer-Bergs reaction conditions. The derivatization proceeded with enough chemoselectivity, minimizing the complication of the reaction system, and thus realizing the facile identification of unnatural hydantoins from the derivatized extracts.
We have also developed new methodology to highlight promiscuous non-specific binding inhibitors of enzymes by interpreting the behavior of concentration-response curves under in vitro DMSO-perturbing assay conditions. Furthermore, we have discovered novel inhibitors of hyaluronan-degrading enzyme from natural plant extracts.
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