Development of extraction techniques for natural products and discovery of hyaluronan-degrading enzyme inhibitors from natural products
| Project/Area Number |
26870217
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General internal medicine(including psychosomatic medicine)
Natural medicines
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| Research Institution | University of Toyama |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 生薬 / 多成分の分子変換 / アミノ酸誘導体 / ヒダントイン / ヒアルロニダーゼ / 化学的摂動効果 / ジメチルスルホキシド / 非特異的分子間相互作用 / 多成分系の分子変換 / ヒアルロン酸分解酵素 / 化学的直接誘導化 / 非特異的阻害剤 / 複雑系 / 生薬成分抽出 / 化学的一挙誘導化 |
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| Outline of Final Research Achievements |
We have achieved direct chemical derivatization of natural plant extracts, where electrophilic ketones in the extracts could be selectively converted into the corresponding hydantoins (imidazolidine-2,4-dione) under the Bucherer-Bergs reaction conditions. The derivatization proceeded with enough chemoselectivity, minimizing the complication of the reaction system, and thus realizing the facile identification of unnatural hydantoins from the derivatized extracts.
We have also developed new methodology to highlight promiscuous non-specific binding inhibitors of enzymes by interpreting the behavior of concentration-response curves under in vitro DMSO-perturbing assay conditions. Furthermore, we have discovered novel inhibitors of hyaluronan-degrading enzyme from natural plant extracts.
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Report
(4 results)
Research Products
(23 results)
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[Presentation] Protective effects of dietary 1,5-Anhydro-D-glucitol in diabetes and metabolic syndrome2015
Author(s)
Atsushi Kato, Keisuke Tomohara, Isao Adachi, Fumihiro Ishikawa, Kei Takeshita, Jacqueline Hollinshead, Robert Nash, Zilei Liu, Sarah Jenkinson, George Fleet
Organizer
2015 International Chemical Congress of Pacific Basin Societies
Place of Presentation
Honolulu
Year and Date
2015-12-15
Related Report
Int'l Joint Research
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