| Project/Area Number |
26870230
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | University of Fukui |
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Principal Investigator |
NAGAOKA Masato 福井大学, テニュアトラック推進本部, 助教 (90397050)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ヒトiPS細胞 / 接着基質 / バイオマテリアル / 分化誘導 / 肝細胞 / 幹細胞 / 肝細胞分化 |
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| Outline of Final Research Achievements |
In this study, defined cell-recognizable materials have been developed for the differentiation of human iPS cells into hepatocyte-like cells. In most of protocols reported previously, Matrigel is used as a substrate for cell adhesion during differentiation. Matrigel is derived from mouse sarcoma, and the method using Matrigel is not a defined condition. First, a small fragment of vitronectin containing RGD sequence was fused with Fc domain of IgG (R-Fc) to establish a defined substrate. This protein supported both maintenance of the pluripotent state of iPS cells and differentiation into hepatocyte-like cells. Next, the patterns of cell-surface protein expression during differentiation were analyzed by DNA array and qPCR to design novel cell adhesive materials for differentiated cells, and specific genes were identified, which expression were up-regulated during differentiation of human iPS cells into hepatic lineage cells.
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