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Development of a theory to describe ion permeation and selectivity through potassium channels

Research Project

Project/Area Number 26870232
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional biochemistry
Physical chemistry
Research InstitutionUniversity of Fukui

Principal Investigator

Sumikama Takashi  福井大学, 医学部, 特命助教 (30579412)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsイオンチャネル / イオン透過 / イオン選択性 / 分子動力学法 / シミュレーション / モデルチャネル / 分子動力学
Outline of Final Research Achievements

Ion selectivity and ion transports through ion channels are essential for life: Living cells generate electrical signals by utilizing selective ion permeation through channels. The aim of this study is to reveal the relationship between ion selectivity and ion permeation. The electrophysiological measurement employing the punch through method implies that the shape of the current-voltage (i-V) curve differs among ion species. In order to explain this difference, the relationship between the shape of i-V curve and the rate-determining step of ion permeation was analyzed by using the molecular dynamics simulation. The shape of the i-V curve of sodium ions through the potassium channel was predicted to be superlinear, which is considered to contribute to the selectivity for potassium over sodium at low voltage.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (10 results)

All 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Origin of the shape of Current-Voltage Curve through Nanopores: A Molecular Dynamics Study2016

    • Author(s)
      Takashi Sumikama
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 25750-25750

    • DOI

      10.1038/srep25750

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Visualization of fluctuating motions of the selectivity filter in the potassium channel: A computational study2015

    • Author(s)
      Takashi Sumikama and Shigetoshi Oiki
    • Journal Title

      The Journal of Physiological Sciences

      Volume: 65

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structure and Dynamics of Membrane-embedded KcsA Potassium Channel Revealed by Atomic Force Microscopy2015

    • Author(s)
      角野歩、山本大輔、炭竃享司、岩本真幸、出羽毅久、老木成稔
    • Journal Title

      Seibutsu Butsuri

      Volume: 55 Issue: 1 Pages: 005-010

    • DOI

      10.2142/biophys.55.005

    • NAID

      130004794734

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Bridging ion permeation and ion selectivity through K+ channel2016

    • Author(s)
      炭竈享司、三田建一郎、老木成稔
    • Organizer
      日本生理学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Difference in ion permeation mechanism through the Kv1.2 and KcsA channels: A molecular dynamics study2015

    • Author(s)
      Takashi Sumikama and Shigetoshi Oiki
    • Organizer
      The International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Hawaii Convention Center
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A kinetic model describing punch-through of Na+ through KcsA potassium channel; イオン透過と選択性を記述する速度論モデル2015

    • Author(s)
      炭竈享司、三田建一郎、老木成稔
    • Organizer
      日本生物物理学会
    • Place of Presentation
      金沢大学角間キャンパス
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] Visualization of fluctuating motions of the selectivity filter in the potassium channel: A computational study2015

    • Author(s)
      炭竈享司, 老木成稔
    • Organizer
      日本生理学会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2015-03-21
    • Related Report
      2014 Research-status Report
  • [Presentation] Fluctuation of carbonyl backbone of the selectivity filter of the Kv1.2 channel during K+ permeation2014

    • Author(s)
      炭竈享司, 老木成稔
    • Organizer
      The 45th NIPS Internaional Symposium, co-sponsored by The Journal of Physiology "Cutting-edge approaches towards the functioning mechanisms of membrane proteins"
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Kv1.2でのイオン透過における透過パターンの解析2014

    • Author(s)
      炭竈享司, 斉藤真司, 老木成稔
    • Organizer
      日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] The Ratio of Flux of Water Molecules over that of Ions through Ion Channel Reflects Interactions between Channel and Ions2014

    • Author(s)
      炭竈享司
    • Organizer
      OIST Workshops "Single Protein Dynamics in Cellulo 2014: Spatio-Temporal, Structural and Quantitative Analyses"
    • Place of Presentation
      OIST, 沖縄
    • Year and Date
      2014-04-21
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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