Elucidation of the flexible structure-function relationship of the potassium channel KcsA using high resolution microscopy

• Project/Area Number: 26870233
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological physics/Chemical physics/Soft matter physics; Biophysics
• Research Institution: University of Fukui
• Principal Investigator: Sumino Ayumi 福井大学, 医学部, 特別研究員 (80717140)
• Research Collaborator: Uchihashi Takayuki 金沢大学, 数物科学系, 教授 (30326300) ; Ulf Skoglund 沖縄科学技術大学院大学, 構造細胞生物学ユニット, 教授
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 生体膜 / 受容体 / チャンネル / 原子間力顕微鏡 / カリウムチャネル / KcsA / 構造変化 / 電子線トモグラフィー

Outline of Final Research Achievements

Structure of pH-dependent potassium channel KcsA is roughly divided into transmembrane and cytoplasmic domains. The high resolution structure of the transmembrane domains have been revealed by the crystal structure, but the high resolution structure of the cytoplasmic domain is unknown. In this study, we aimed to elucidate the structure of the cytoplasmic domain of KcsA channel in water and non-crystalline state. Atomic force microscopy revealed that, the cytoplasmic domain of KcsA channel were fluctuating at a high speed. The result indicates that the cytoplasmic domain of KcsA does not take a hard specific structure in water.

Research Products

• [Journal Article] Structure and Dynamics of Membrane-embedded KcsA Potassium Channel Revealed by Atomic Force Microscopy (2015)
  • Author(s): 角野歩、山本大輔、炭竃享司、岩本真幸、出羽毅久、老木成稔
  • Journal Title: Seibutsu Butsuri Volume: 55 Issue: 1 Pages: 005-010
  • DOI: 10.2142/biophys.55.005
  • NAID: 130004794734
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 高速原子間力顕微鏡で見るカリウムチャネルKcsAの動的挙動 (2015)
  • Author(s): Ayumi Sumino, Takayuki Uchihashi, Daisuke Yamamoto, Masayuki Iwamoto, Takehisa Dewa, Shigetoshi Oiki
  • Organizer: 第92回日本生理学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2015-03-21
• [Presentation] Gating-associated clustering–dispersion dynamics of the KcsA potassium channel in a lipid membrane: Direst observation using atomic force microscopy (2014)
  • Author(s): Sumino, Ayumi; Yamamoto, Daisuke; Iwamoto, Masayuki; Dewa, Takehisa; Oiki, Shigetoshi
  • Organizer: The 45th NIPS Internaional Symposium, co-sponsored by The Journal of Physiology
  • Place of Presentation: 岡崎カンファレンスセンター
  • Year and Date: 2014-11-26 – 2014-11-28
• [Presentation] カリウムチャネルKcsAのゲート開閉と連動した膜中集合・離散 (2014)
  • Author(s): 角野 歩、山本 大輔、岩本 真幸、出羽 毅久、老木 成稔
  • Organizer: 第５２回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-25
• [Presentation] 原子間力顕微鏡による生体膜研究へのアプローチ (2014)
  • Author(s): 角野 歩
  • Organizer: アサイラムAFMセミナー「blueDrive フォトサーマル励振技術」
  • Place of Presentation: AP大阪駅前梅田1丁目 B1F Eルーム
  • Year and Date: 2014-08-01
  • Invited
• [Presentation] カリウムチャネルKcsAのゲート開閉と連動した膜中集合離散：原子間力顕微鏡で明らかになった集団的振る舞い (2014)
  • Author(s): 角野 歩、山本 大輔、岩本 真幸、出羽 毅久、老木 成稔
  • Organizer: 第24回 バイオ･高分子シンポジウム
  • Place of Presentation: 東京工業大学大岡山キャンパス
  • Year and Date: 2014-07-14
• [Presentation] Gating-associated clustering-dispersion behavior of the KcsA potassium channel in membrane: Direct observation by atomic force microscopy (2014)
  • Author(s): Sumino, Ayumi; Yamamoto, Daisuke; Iwamoto, Masayuki; Dewa, Takehisa; Oiki, Shigetoshi
  • Organizer: Single Protein Dynamics in Cellulo 2014: Spatio-Temporal, Structural and Quantitative Analyses:
  • Place of Presentation: 沖縄科学技術大学院大学シーサイドハウス
  • Year and Date: 2014-04-10