New biochemical methods for plant enzymatic activity

• Project/Area Number: 26870252
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry; Biofunction/Bioprocess
• Research Institution: Shizuoka University
• Principal Investigator: Ohnishi Toshiyuki 静岡大学, 農学部, 准教授 (60542165)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: テルペン / 遺伝子導入 / タバコ / インフィルトレーション法

Outline of Final Research Achievements

We constructed a new methods for biochemical identification of plant enzymes using Nicotiana benthamiana as heterologous expression platform. 1-Deoxy-D-xylulose 5-phosphate gene and monoterpene synthases from Camellia sinensis installed in Nicotiana benthamiana gave higher amount of monoterenoids rather than wild type Nicotiana benthamiana. This new methods would open the door to identification of plant terpene synthases without neither adding substrate candidates nor extracting enzymes.

Research Products

• [Int'l Joint Research] ミシガン州立大学(米国)
• [Journal Article] 茶の香気成分の貯蔵メカニズム 茶は香りをどのように繋ぎとめるのか？ (2016)
  • Author(s): 大西 利幸, 小埜 栄一郎
  • Journal Title: 日本家政学会誌 Volume: ６７ Pages: 238-242
  • NAID: 130005144764
  • Open Access
• [Journal Article] 茶の香り成分の貯蔵に関わる配糖体化酵素の解明 (2016)
  • Author(s): 大西 利幸, 小埜 栄一郎
  • Journal Title: イオサイエンスとインダストリー(B&I) Volume: 74 Pages: 129-131
  • NAID: 40020762019
  • Open Access
• [Journal Article] Volatile Glycosylation in Tea Plants: Sequential Glycosylations for the Biosynthesis of Aroma β-Primeverosides Are Catalyzed by Two Camellia sinensis Glycosyltransferases (2015)
  • Author(s): Shoji Ohgami, Eiichiro Ono, Manabu Horikawa, Jun Murata, Koujirou Totsuka, Hiromi Toyonaga, Yukie Ohba, Hideo Dohra, Tatsuo Asai, Kenji Matsui, Masaharu Mizutani, Naoharu Watanabe and Toshiyuki Ohnishi
  • Journal Title: Plant Physiology Volume: 168 Pages: 464-477
  • NAID: 120005616190
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 環境変動に対するチャの 生体防御システムの解析 (2017)
  • Author(s): 大西 利幸
  • Organizer: ナノ・マイクロ横断型人材育成シンポジウム
  • Place of Presentation: 静岡大学 浜松キャンパス (静岡県，浜松市)
  • Invited
• [Presentation] お茶の香りの生成・貯蔵メカニズム (2016)
  • Author(s): 大西 利幸
  • Organizer: 第三回 メタボローム勉強会
  • Place of Presentation: 鶴岡メタボロームキャンパス (山形県鶴岡市)
  • Year and Date: 2016-02-13
  • Invited
• [Presentation] お茶の“甘い”香りとその貯蔵の仕組み (2016)
  • Author(s): 大西 利幸
  • Organizer: 日本農芸化学会中四国支部創立15周年記念 第３１回 市民フォーラム
  • Place of Presentation: カリエンテ山口 (山口県，山口市)
  • Invited
• [Presentation] お茶の“甘い”香りとその貯蔵の仕組み (2016)
  • Author(s): 大西 利幸
  • Organizer: 高大連携出張授業
  • Place of Presentation: 静岡市立高校 (静岡県，静岡市)
  • Invited
• [Presentation] 「香り」が寄与する植物の化学防御システム チャの香り成分を貯蔵するための二つの配糖化酵素 (2016)
  • Author(s): 大西 利幸
  • Organizer: 平成28年東海地区化学教育討論会
  • Place of Presentation: B-NEST (静岡県，静岡市)
  • Invited
• [Presentation] お茶の香りの貯蔵メカニズム ～香り成分の安定的な貯蔵の鍵を握る酵素遺伝子～ (2016)
  • Author(s): 大西 利幸
  • Organizer: 大阪府立大学応用生命科学セミナー
  • Place of Presentation: 大阪府立大学 (大阪府，堺市)
  • Invited
• [Presentation] お茶の香りの貯蔵メカニズム～香り成分の安定的な貯蔵の鍵を握る酵素遺伝子～ (2015)
  • Author(s): 大西 利幸
  • Organizer: 第35回C-Bioセミナー
  • Place of Presentation: 宇都宮大学 (栃木県宇都宮市)
  • Year and Date: 2015-09-17
  • Invited
• [Presentation] Sequential glycosylations for biosynthesis of tea aroma primeverosides are catalyzed via two glycosyltransferases (2015)
  • Author(s): Shoji Ohgami, Eiichiro Ono, Manabu Horikawa, Jun Murata, Hiromi Toyonaga, Hideo Dohra, Masaharu Mizutani, Watanabe Naoharu and Toshiyuki Ohnishi
  • Organizer: 12th International Meeting on Biosynthesis, Function and Synthetic Biology of Isoprenoids.
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2015-06-01
  • Int'l Joint Research
• [Book] Plant Specialized Metabolism: Genomics, Biochemistry, and Biological Functions (2016)
  • Author(s): Eiichiro Ono and Toshiyuki Ohnishi
  • Total Pages: 376
  • Publisher: CRC Press, Taylor & Francis Group,