New biochemical methods for plant enzymatic activity
Project/Area Number |
26870252
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied biochemistry
Biofunction/Bioprocess
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Research Institution | Shizuoka University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | テルペン / 遺伝子導入 / タバコ / インフィルトレーション法 |
Outline of Final Research Achievements |
We constructed a new methods for biochemical identification of plant enzymes using Nicotiana benthamiana as heterologous expression platform. 1-Deoxy-D-xylulose 5-phosphate gene and monoterpene synthases from Camellia sinensis installed in Nicotiana benthamiana gave higher amount of monoterenoids rather than wild type Nicotiana benthamiana. This new methods would open the door to identification of plant terpene synthases without neither adding substrate candidates nor extracting enzymes.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Volatile Glycosylation in Tea Plants: Sequential Glycosylations for the Biosynthesis of Aroma β-Primeverosides Are Catalyzed by Two Camellia sinensis Glycosyltransferases2015
Author(s)
Shoji Ohgami, Eiichiro Ono, Manabu Horikawa, Jun Murata, Koujirou Totsuka, Hiromi Toyonaga, Yukie Ohba, Hideo Dohra, Tatsuo Asai, Kenji Matsui, Masaharu Mizutani, Naoharu Watanabe and Toshiyuki Ohnishi
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Journal Title
Plant Physiology
Volume: 168
Pages: 464-477
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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