Biochemical analysis of novel clock associated genes

• Project/Area Number: 26870267
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry; Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: Nakamichi Norihito 名古屋大学, 理学研究科(WPI), 准教授 (90513440)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 植物 / 概日時計 / 概日リズム / 転写因子 / 転写ネットワーク / 転写制御 / ネットワーク / 生物時計 / クロマチン免疫沈降 / シロイヌナズナ

Outline of Final Research Achievements

Circadian rhythms is an endogenous oscillation with a period about 24 hr. In order to understand molecular mechanism underlying circadian clock in plants, we performed functional genomic approaches. This project found that Arabidopsis CCA1, a morning-expressed clock-associated transcriptional factor, directly regulates a set of clock-associated genes expressed in the evening. Furthermore, CCA1 directly regulates key genes involved in environmental stress responses. These genes are expressed in the evening. This study illustrates that direct binding by CCA1 in the morning defines strong repression in a genomic scale.

Research Products

• [Journal Article] Direct repression of evening genes by CIRCADIAN CLOCK-ASSOCIATED1 in the Arabidopsis circadian clock. (2016)
  • Author(s): Kamioka M, Takao S, Suzuki T, Taki K, Higashiyama T, Kinoshita T, Nakamichi N
  • Journal Title: Plant Cell Volume: 28 Issue: 3 Pages: 696-711
  • DOI: 10.1105/tpc.15.00737
  • Peer Reviewed / Open Access
• [Journal Article] Improvement of Arabidopsis biomass and cold-, drought-, and salinity-stress tolerance by modified circadian clock-associated PSEUDO-RESPONSE REGULATORs. (2016)
  • Author(s): Nakamichi N, Takao S, Kudo T, Kiba T, Wang Y, Kinoshita T, Sakakibara H.
  • Journal Title: Plant Cell Physiol. Volume: 57
  • NAID: 120005898470
  • Peer Reviewed
• [Journal Article] Adaptation to the Local Environment by Modifications of the Photoperiod Response in Crops. (2015)
  • Author(s): Nakamichi N
  • Journal Title: Plant Cell Physiol. Volume: 56 Issue: 4 Pages: 594-604
  • DOI: 10.1093/pcp/pcu181
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A flowering integrator, SOC1, affects stomatal opening in Arabidopsis thaliana. (2015)
  • Author(s): Yuriko Kimura, Saya Aoki, Eigo Ando, Ayaka Kitatsuji, Aiko Watanabe, Masato Ohnishi, Koji Takahashi, Shin-ichiro Inoue, Norihito Nakamichi, Yosuke Tamada, Toshinori Kinoshita
  • Journal Title: Plant Cell Physiology Volume: 56 Issue: 4 Pages: 640-649
  • DOI: 10.1093/pcp/pcu214
  • Peer Reviewed / Open Access
• [Journal Article] The LNK1 night light-inducible and clock-regulated gene is induced also in response to warm-night through the circadian clock nighttime repressor in Arabidopsis thaliana. (2014)
  • Author(s): Mizuno T, Takeuchi A, Nomoto Y, Nakamichi N, Yamashino T.
  • Journal Title: Plant Signal Behav. Volume: 9
  • Open Access
• [Presentation] Synthetic Molecules Changing Plant Circadian Clock (2016)
  • Author(s): 中道範人
  • Organizer: The 64th NIBB Conference Evolution of Seasonal Timers
  • Place of Presentation: 基礎生物学研究所
  • Year and Date: 2016-04-22
  • Int'l Joint Research / Invited
• [Presentation] 概日リズムを長周期化する低分子化合物のターゲットとして同定されたキナーゼの解析 (2016)
  • Author(s): 水谷佳幸, 上原貴大, 山口潤一郎, 高尾早織, 瀧京美, 佐藤綾人, 桑田啓子, 伊丹健一郎, 木下俊則, 中道範人
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡
  • Year and Date: 2016-03-18
• [Presentation] 概日リズムの周期長を変える化合物の作用機序の理解に向けた遺伝学的解析 (2016)
  • Author(s): 瀧京美, 上原貴大, 山口潤一郎, 高尾早織, 笠原博幸, 伊丹健一郎, 木下俊則, 中道範人
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡
  • Year and Date: 2016-03-18
• [Presentation] 植物の概日時計機能に関わるタンパク質PRRの研究 (2015)
  • Author(s): 中道範人
  • Organizer: 第22回日本時間生物学会学術大会
  • Place of Presentation: 東京大学
  • Year and Date: 2015-11-21
  • Invited
• [Presentation] Small molecules controlling Arabidopsis circadian rhythms. (2015)
  • Author(s): Nakamichi N.
  • Organizer: The 56th Annual Meeting of the Japanese Society of Plant Physiologists
  • Place of Presentation: Tokyo
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Genetic architecture of Arabidopsis circadian clock system (2014)
  • Author(s): Nakamichi N.
  • Organizer: The 37th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Yokohama
  • Year and Date: 2014-11-25 – 2014-11-27
  • Invited
• [Presentation] Systematic approaches to understand plant circadian clock system, (2014)
  • Author(s): Nakamichi N.
  • Organizer: The 38th Naito conference
  • Place of Presentation: Sapporo
  • Year and Date: 2014-10-07 – 2014-10-10
• [Presentation] Synthetic molecules controlling plant circadian rhythms (2014)
  • Author(s): Nakamichi N.
  • Organizer: 2nd International Symposium of Transformative Bio-molecules,
  • Place of Presentation: Nagoya
  • Year and Date: 2014-05-12
  • Invited
• [Patent(Industrial Property Rights)] 植物概日リズム調整剤。 (2014)
  • Inventor(s): 中道範人
  • Industrial Property Rights Holder: 名古屋大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-08-12